Transcription termination on the coliphage lambda chromosome is suppressed by the viral N protein. Host mutations which block N action (nus, groN) also affect transcription termination on the bacterial chromosome. We find that nusB mutants are defective in transcribing ribosomal RNA; we are selecting additional E. coli mutants with this defect. We have designed a novel lambda vector to perform this selection, and to verify that transcription termination is affected in these mutants. Other E. coli mutations that alter transcription termination have been isolated as suppressors of the nusAl phenotype. One such suppressor appears to lie in gene """"""""u"""""""", which encodes a 21 kd protein and is located in a cluster of genes involved in mRNA translation. We wish to determine the nature of the """"""""u"""""""" product, and to ask if it serves as a link between transcription and translation. Bacteriophage lambda does not propagate on E. coli lysogenic for phage HK022. This exclusion is due to a HK022 prophage function, Nun, which acts as a new transcription termination factor. Nun interacts with Nus factors to provoke premature termination at the lambda nut sites.
| Saxena, Shivalika; Myka, Kamila K; Washburn, Robert et al. (2018) Escherichia coli transcription factor NusG binds to 70S ribosomes. Mol Microbiol 108:495-504 |
| Gottesman, Max E; Mustaev, Arkady (2018) Inorganic phosphate, arsenate, and vanadate enhance exonuclease transcript cleavage by RNA polymerase by 2000-fold. Proc Natl Acad Sci U S A 115:2746-2751 |
| Zuber, Philipp K; Hahn, Lukas; Reinl, Anne et al. (2018) Structure and nucleic acid binding properties of KOW domains 4 and 6-7 of human transcription elongation factor DSIF. Sci Rep 8:11660 |
| Schrank, Benjamin R; Aparicio, Tomas; Li, Yinyin et al. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature 559:61-66 |
| Kang, Jin Young; Olinares, Paul Dominic B; Chen, James et al. (2017) Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex. Elife 6: |
| Mustaev, Arkady; Roberts, Jeffrey; Gottesman, Max (2017) Transcription elongation. Transcription 8:150-161 |
| Strauß, Martin; Vitiello, Christal; Schweimer, Kristian et al. (2016) Transcription is regulated by NusA:NusG interaction. Nucleic Acids Res 44:5971-82 |
| Mustaev, Arkady; Vitiello, Christal L; Gottesman, Max E (2016) Probing the structure of Nun transcription arrest factor bound to RNA polymerase. Proc Natl Acad Sci U S A 113:8693-8 |
| Washburn, Robert S; Gottesman, Max E (2015) Regulation of transcription elongation and termination. Biomolecules 5:1063-78 |
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