In order to understand the regulation of the transcription and translation of C Mu and C Delta immunoglobulin genes, we plan to first evaluate the rate of synthesis of mRNA for Mu and Delta chains in a B lymphoma cell line BCL1, which expresses both isotypes. Secondly, since BCL1 cells can be induced to differentiate to immunoglobulin secretion by stimulation with lipopolysaccharide (LPS), we will also measure the effect of this perturbance of the steady state on the rate of mRNA synthesis for the two species. Thirdly, we will examine the rates of IgM and IgD synthesis before and after LPS stimulation to determine whether translational and/or post-translational control also play a role in the regulation of the synthesis of these two isotypes. Finally, as a first step towards analysis of the termination and/or processing enzymes involved in producton of the various mature Mu and Delta mRNA species, experiments will be conducted to detect the primary transcriptional products of C Mu and C Delta genes. The results of these experiments should contribute significantly towards our understanding of the relationship of the two transcriptional units and the effect of B cell activation on these units.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM037743-05
Application #
3293377
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1986-05-01
Project End
1989-04-30
Budget Start
1987-05-01
Budget End
1988-04-30
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390
Yuan, D; Goldings, E A (1988) Transient expression of pentameric IgM on the surface of NZB B cells. Mol Immunol 25:323-8