Abstract

The goal of this work is to understand, at the molecular level, the mechanisms of mother cell specific transcriptional regulation in Saccharomyces cerevisiae. The yeast HO gene is transcribed only in mother cells, one of the two cell types of cells descending from each mitotic division. An analogy can be drawn between this lineage specificity in HO expression and the differential gene regulation which occurs during embryonic development. Two proteins, SWI5 and SDI1, have been identified which bind to the HO promoter and are also involved in determining the mother cell specificity of HO gene expression. The interactions of these proteins with DNA and with each other will be characterized in detail. This information will be used to develop specific models of transcriptional regulation, and these models will be tested by in vitro and in vivo experiments. Additional genes regulated by SWI5 and SDI1 will be cloned from the yeast genome. The regulatory properties of these genes will be determined, and the promoters will be characterized for DNA sites recognized by these two site-specific DNA-binding proteins. Further information on the mechanism of mother cell specific transcription will be available by comparing these genes with HO, specifically by analyzing their protein-DNA interactions and their transcriptional regulation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM039067-03
Application #
3295893
Study Section
Molecular Biology Study Section (MBY)
Project Start
1988-02-01
Project End
1991-01-31
Budget Start
1990-02-01
Budget End
1991-01-31
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Yarrington, Robert M; Goodrum, Jenna M; Stillman, David J (2016) Nucleosomes Are Essential for Proper Regulation of a Multigated Promoter in Saccharomyces cerevisiae. Genetics 202:551-63
Yu, Yaxin; Yarrington, Robert M; Chuong, Edward B et al. (2016) Disruption of promoter memory by synthesis of a long noncoding RNA. Proc Natl Acad Sci U S A 113:9575-80
Yarrington, Robert M; Rudd, Jared S; Stillman, David J (2015) Spatiotemporal cascade of transcription factor binding required for promoter activation. Mol Cell Biol 35:688-98
Zapata, Jessica; Dephoure, Noah; Macdonough, Tracy et al. (2014) PP2ARts1 is a master regulator of pathways that control cell size. J Cell Biol 204:359-76
Parnell, Emily J; Yu, Yaxin; Lucena, Rafael et al. (2014) The Rts1 regulatory subunit of PP2A phosphatase controls expression of the HO endonuclease via localization of the Ace2 transcription factor. J Biol Chem 289:35431-7
Voth, Warren P; Takahata, Shinya; Nishikawa, Joy L et al. (2014) A role for FACT in repopulation of nucleosomes at inducible genes. PLoS One 9:e84092
Stillman, David J (2013) Dancing the cell cycle two-step: regulation of yeast G1-cell-cycle genes by chromatin structure. Trends Biochem Sci 38:467-75
Zhang, Qian; Yoon, Youngdae; Yu, Yaxin et al. (2013) Stochastic expression and epigenetic memory at the yeast HO promoter. Proc Natl Acad Sci U S A 110:14012-7
Tantin, Dean; Voth, Warren P; Shakya, Arvind (2013) Efficient chromatin immunoprecipitation using limiting amounts of biomass. J Vis Exp :e50064
Stillman, David J (2010) Nhp6: a small but powerful effector of chromatin structure in Saccharomyces cerevisiae. Biochim Biophys Acta 1799:175-80

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