Membrane proteins make up over 30% of the human proteome and are critical in biological functions, including transport and the transduction of cellular information. Membrane proteins also feature in the multistep pathways that lead to glycoproteins. For example, the human pathway for protein N-glycosylation, occurs exclusively at cellular membranes and is part of the essential process that ultimately affords all cell- surface and secreted N-linked glycoproteins. Likewise, bacterial, glycoproteins are generated through stepwise, membrane-associated glycan assembly pathways, which culminate in the biosynthesis of important virulence-associated glycoproteins. Despite the widespread importance of multistep membrane pathways in human health and disease and the extensive knowledge on the enzymes that make up these pathways, our understanding of how the enzymes function together and in an ordered sequence are greatly challenged by technical issues associated with the amphiphilic nature of membranes and the properties of associated membrane-bound proteins. Functional studies on membrane proteins are often simplified by extraction into detergent micelles. However, this treatment is highly perturbing and under these conditions, all but the most stable multiprotein complexes will dissociate and the cryptic information that is programmed in the native membrane will be lost. Therefore, a major current challenge is to develop strategies for understanding how proteins are recruited into functional complexes at cellular membranes. This challenge demands the application of synergistic in vivo and in vitro experimental approaches. The proposed research will investigate the membrane-associated protein N-glycosylation pathway of the Gram-negative enteropathogen Campylobacter jejuni, which shares the logic of the more complex mammalian pathway. The research has three aims.
In Aim 1 we will define the membrane protein interactome for bacterial N-glycosylation using styrene maleic acid lipoparticles (SMALP). SMALP will enable definition of a complete molecular description of the local membrane environment around target membrane proteins in vivo.
In Aim 2 we will leverage the interactome information for in vitro studies in lipid bilayer Nanodiscs (NDs), which provide a native-like model membrane of defined composition, and enable in vitro experimental approaches to understand the rules defining the membrane protein interactions and coordinated function.
In Aim 3 we will implement crosslinking studies to address a key question concerning the timing of glycan transfer to protein. Ultimately, the studies will deliver detailed information regarding the membrane environment and protein interaction network that supports efficient N-glycosylation in a representative bacterial pathway. If successful, the research will provide insight into the molecular logic underpinning the processes that lead to glycoprotein biosynthesis in all living systems and will inform on multidisciplinary approaches for investigating other physiologically significant multienzyme processes that occur at membrane interfaces.

Public Health Relevance

Biological membranes are self-assembled structures that are found within and around the cells of all living organisms. Rather than simply representing barriers to establish boundaries, these essential dynamic structures provide a unique environment for many important multi- step biological pathways. This grant will elucidate the details of the membrane-associated manufacturing process that leads to glycoproteins, which ultimately participate in fundamental biological processes ranging from tissue and organ development to bacterial infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM039334-32
Application #
9853792
Study Section
Macromolecular Structure and Function A Study Section (MSFA)
Program Officer
Bond, Michelle Rueffer
Project Start
1988-02-01
Project End
2021-01-31
Budget Start
2020-02-01
Budget End
2021-01-31
Support Year
32
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02142
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Eichler, Jerry; Imperiali, Barbara (2018) Biogenesis of Asparagine-Linked Glycoproteins Across Domains of Life-Similarities and Differences. ACS Chem Biol 13:833-837
Ray, Leah C; Das, Debasis; Entova, Sonya et al. (2018) Membrane association of monotopic phosphoglycosyl transferase underpins function. Nat Chem Biol 14:538-541
Eichler, Jerry; Imperiali, Barbara (2018) Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases. Trends Biochem Sci 43:10-17
Das, Debasis; Kuzmic, Petr; Imperiali, Barbara (2017) Analysis of a dual domain phosphoglycosyl transferase reveals a ping-pong mechanism with a covalent enzyme intermediate. Proc Natl Acad Sci U S A 114:7019-7024
Lukose, Vinita; Walvoort, Marthe T C; Imperiali, Barbara (2017) Bacterial phosphoglycosyl transferases: initiators of glycan biosynthesis at the membrane interface. Glycobiology 27:820-833
Musial-Siwek, Monika; Jaffee, Marcie B; Imperiali, Barbara (2016) Probing Polytopic Membrane Protein-Substrate Interactions by Luminescence Resonance Energy Transfer. J Am Chem Soc 138:3806-12
Das, Debasis; Walvoort, Marthe T C; Lukose, Vinita et al. (2016) A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyltransferase Enzymes. Sci Rep 6:33412
Silverman, Julie Michelle; Imperiali, Barbara (2016) Bacterial N-Glycosylation Efficiency Is Dependent on the Structural Context of Target Sequons. J Biol Chem 291:22001-22010
Whitworth, Garrett E; Imperiali, Barbara (2015) Selective biochemical labeling of Campylobacter jejuni cell-surface glycoconjugates. Glycobiology 25:756-66

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