Abstract

Several regulators of meiosis in S cerevisiae act on the Rim101 pathway, a conserved signal transduction pathway. Homologous pathways promote expression of genes in response to external alkaline pH in other fungi and, as these investigators have recently found, in yeast as well. External pH is a biological signal that governs growth, physiology, and differentiation of virtually all cells, yet there is limited information about the transcriptional responses to external pH changes. The long-term objective of this study is to determine how external pH changes govern responses at the level of gene expression and cellular differentiation. The immediate goal is to define the mechanisms of signal transduction through and output of the Rim101 pathway, and to use this information to develop a framework for understanding the pH response and its relationship to meiosis.
Specific aims are to determine the functional relationships among Rim101 pathway members, to determine how the Rim101 pathway governs target gene expression, and to determine the relationship between the Rim101 pathway and functional suppressor genes that can promote alkaline pH responses and meiosis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM039531-15
Application #
6498667
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Anderson, James J
Project Start
1988-02-03
Project End
2005-01-31
Budget Start
2002-02-01
Budget End
2003-01-31
Support Year
15
Fiscal Year
2002
Total Cost
$389,171
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032

  Publications

Subramanian, Shoba; Woolford, Carol A; Desai, Jigar V et al. (2012) cis- and trans-acting localization determinants of pH response regulator Rim13 in Saccharomyces cerevisiae. Eukaryot Cell 11:1201-9
Morohashi, Nobuyuki; Nakajima, Kumiko; Kurihara, Daichi et al. (2007) A nucleosome positioned by alpha2/Mcm1 prevents Hap1 activator binding in vivo. Biochem Biophys Res Commun 364:583-8
Boysen, Jacob H; Mitchell, Aaron P (2006) Control of Bro1-domain protein Rim20 localization by external pH, ESCRT machinery, and the Saccharomyces cerevisiae Rim101 pathway. Mol Biol Cell 17:1344-53
Morohashi, Nobuyuki; Yamamoto, Yuichi; Kuwana, Shunsuke et al. (2006) Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome. Eukaryot Cell 5:1925-33
Barwell, Karen J; Boysen, Jacob H; Xu, Wenjie et al. (2005) Relationship of DFG16 to the Rim101p pH response pathway in Saccharomyces cerevisiae and Candida albicans. Eukaryot Cell 4:890-9
Xu, Wenjie; Smith Jr, Frank J; Subaran, Ryan et al. (2004) Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans. Mol Biol Cell 15:5528-37
Mitchell, Aaron P (2003) Updated view of Cryptococcus neoformans mating type and virulence. Infect Immun 71:4829-30
Lamb, Teresa M; Mitchell, Aaron P (2003) The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol Cell Biol 23:677-86
Shimizu, Mitsuhiro; Takahashi, Keiko; Lamb, Teresa M et al. (2003) Yeast Ume6p repressor permits activator binding but restricts TBP binding at the HOP1 promoter. Nucleic Acids Res 31:3033-7
Blumental-Perry, Anna; Li, Weishi; Simchen, Giora et al. (2002) Repression and activation domains of RME1p structurally overlap, but differ in genetic requirements. Mol Biol Cell 13:1709-21

Showing the most recent 10 out of 25 publications