Abstract

The applicant will use molecular genetic, imaging and biophysical techniques to determine how the actin filament network is formed and shaped by actin cross-linking proteins. Mutant cell lines which contain alterations in genes coding for the actin binding proteins ABP-120, ABP-240 and a-actinin as well as myosin II will be studied. Several novel in vitro and in vivo assays will be used to assess the consequences of these alterations in the cytoskeleton.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM040599-11
Application #
6385744
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Deatherage, James F
Project Start
1988-07-01
Project End
2003-12-14
Budget Start
2001-04-01
Budget End
2003-12-14
Support Year
11
Fiscal Year
2001
Total Cost
$225,968
Indirect Cost

  Institution

Name
University of Connecticut
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
Storrs-Mansfield
State
CT
Country
United States
Zip Code
06269

  Publications

Lemieux, Michael G; Janzen, Dani; Hwang, Rander et al. (2014) Visualization of the actin cytoskeleton: different F-actin-binding probes tell different stories. Cytoskeleton (Hoboken) 71:157-69
Knecht, David A; LaFleur, Rebecca A; Kahsai, Alem W et al. (2010) Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics. PLoS One 5:e14039
Gilberti, Renee M; Joshi, Gaurav N; Knecht, David A (2008) The phagocytosis of crystalline silica particles by macrophages. Am J Respir Cell Mol Biol 39:619-27
Lombardi, M L; Knecht, D A; Lee, J (2008) Mechano-chemical signaling maintains the rapid movement of Dictyostelium cells. Exp Cell Res 314:1850-9
Washington, Raymond W; Knecht, David A (2008) Actin binding domains direct actin-binding proteins to different cytoskeletal locations. BMC Cell Biol 9:10
Tang, Linnan; Franca-Koh, Jonathan; Xiong, Yuan et al. (2008) tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis. Genes Dev 22:2278-90
Hadjout, Nacima; Yin, Xiuyun; Knecht, David A et al. (2007) Automated real-time measurements of leukocyte chemotaxis. J Immunol Methods 320:70-80
Yin, Xiuyun; Knecht, David A; Lynes, Michael A (2005) Metallothionein mediates leukocyte chemotaxis. BMC Immunol 6:21
Gao, Tong; Knecht, David; Tang, Lei et al. (2004) A cell number counting factor regulates Akt/protein kinase B to regulate Dictyostelium discoideum group size. Eukaryot Cell 3:1176-84
Thibodeau, Michael S; Giardina, Charles; Knecht, David A et al. (2004) Silica-induced apoptosis in mouse alveolar macrophages is initiated by lysosomal enzyme activity. Toxicol Sci 80:34-48

Showing the most recent 10 out of 38 publications