Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM040635-07
Application #
2180492
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1988-07-01
Project End
1996-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
7
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Vu, H M; Liu, W; Grove, A et al. (2000) Mechanisms for the enhanced thermal stability of a mutant of transcription factor 1 as explained by (1)H, (15)N and (13)C NMR chemical shifts and secondary structure analysis. Biochim Biophys Acta 1478:113-24
Vu, H M; Pepe, A; Mayol, L et al. (1999) NMR-derived solution structure of a 17mer hydroxymethyluracil-containing DNA. Nucleic Acids Res 27:4143-50
Vu, H M; Pasternack, L B; Kearns, D R (1999) Specificity of hydroxylmethyluracil-containing DNA for transcription factor 1: structural insights. Biopolymers 52:57-63
Silva, M V; Pasternack, L B; Kearns, D R (1997) Nuclear magnetic resonance-based model of a TF1/HmU-DNA complex. Arch Biochem Biophys 348:255-61
Pasternack, L B; Bramham, J; Mayol, L et al. (1996) 1H NMR studies of the 5-(hydroxymethyl)-2'-deoxyuridine containing TF1 binding site. Nucleic Acids Res 24:2740-5
Jia, X; Grove, A; Ivancic, M et al. (1996) Structure of the Bacillus subtilis phage SPO1-encoded type II DNA-binding protein TF1 in solution. J Mol Biol 263:259-68
Hsu, V L; Jia, X; Kearns, D R (1995) Multidimensional NMR spectroscopy of DNA-binding proteins: structure and function of a transcription factor. Toxicol Lett 82-83:577-89
Jia, X; Reisman, J M; Hsu, V L et al. (1994) Proton and nitrogen NMR sequence-specific assignments and secondary structure determination of the Bacillus subtilis SPO1-encoded transcription factor 1. Biochemistry 33:8842-52
Brown, D R; Kurz, M; Kearns, D R et al. (1994) Formation of multiple complexes between actinomycin D and a DNA hairpin: structural characterization by multinuclear NMR. Biochemistry 33:651-64
Reisman, J M; Hsu, V L; Jariel-Encontre, I et al. (1993) A 1H-NMR study of the transcription factor 1 from Bacillus subtilis phage SPO1 by selective 2H-labeling. Complete assignment and structural analysis of the aromatic resonances for a 22-kDa homodimer. Eur J Biochem 213:865-73

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