Abstract

Dr. Michaelis is studying the biogenesis of the S. Cerevisiae mating pheromone a-factor. She will explore the hypothesis that a-factor biogenesis may be carried out by a modular or multicomponent """"""""a-factor biogenesis machine. Her long-term goal is to define at high resolution the dynamic events that occur during a-factor biogenesis and to understand how these events are coordinated and regulated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM041223-08A1
Application #
2396056
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1988-12-01
Project End
2001-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
8
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Worman, Howard J; Michaelis, Susan (2018) Permanently Farnesylated Prelamin A, Progeria, and Atherosclerosis. Circulation 138:283-286
Spear, Eric D; Hsu, Erh-Ting; Nie, Laiyin et al. (2018) ZMPSTE24 missense mutations that cause progeroid diseases decrease prelamin A cleavage activity and/or protein stability. Dis Model Mech 11:
Ast, Tslil; Michaelis, Susan; Schuldiner, Maya (2016) The Protease Ste24 Clears Clogged Translocons. Cell 164:103-114
Mehmood, Shahid; Marcoux, Julien; Gault, Joseph et al. (2016) Mass spectrometry captures off-target drug binding and provides mechanistic insights into the human metalloprotease ZMPSTE24. Nat Chem 8:1152-1158
Kane, Megan S; Lindsay, Mark E; Judge, Daniel P et al. (2013) LMNA-associated cardiocutaneous progeria: an inherited autosomal dominant premature aging syndrome with late onset. Am J Med Genet A 161A:1599-611
Michaelis, Susan; Hrycyna, Christine A (2013) Biochemistry. A protease for the ages. Science 339:1529-30
Barrowman, Jemima; Wiley, Patricia A; Hudon-Miller, Sarah E et al. (2012) Human ZMPSTE24 disease mutations: residual proteolytic activity correlates with disease severity. Hum Mol Genet 21:4084-93
Michaelis, Susan; Barrowman, Jemima (2012) Biogenesis of the Saccharomyces cerevisiae pheromone a-factor, from yeast mating to human disease. Microbiol Mol Biol Rev 76:626-51
Barrowman, Jemima; Hamblet, Corinne; Kane, Megan S et al. (2012) Requirements for efficient proteolytic cleavage of prelamin A by ZMPSTE24. PLoS One 7:e32120
Barrowman, Jemima; Bhandari, Deepali; Reinisch, Karin et al. (2010) TRAPP complexes in membrane traffic: convergence through a common Rab. Nat Rev Mol Cell Biol 11:759-63

Showing the most recent 10 out of 36 publications