While chromatin provides a means for packaging large amounts of DNA inside a small volume, it is also the substrate for key processes such as replication, recombination and transcription. It has become increasingly apparent during past decade that eukaryotic cells often control these processes by mechanisms that depend upon chromatin structure. Genes can be activated or silenced by factors that changes the position of nucleosomes around enhancers and promoter. Chromatin modifications may also influence gene expression. For example, hyperactivation of X-linked gene expression in male flies is accompanied by an increase in histone H4 acetylation, while silencing of the mating type loci in yeast is accompanied by deacetylation The research described in this proposal focuses on regulatory elements that appear to exert their effects by chromatin dependent mechanisms. Two different class of regulatory elements will be examined. The first are domain boundary elements or insulators, while the second are Polycomb dependent silencers or PREs.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM043432-11
Application #
6180287
Study Section
Genetics Study Section (GEN)
Program Officer
Carter, Anthony D
Project Start
1989-12-01
Project End
2003-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
11
Fiscal Year
2000
Total Cost
$297,715
Indirect Cost
Name
Princeton University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
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