The long-term goal of this research is to understand the mechanisms that regulate the assembly, targeting and activity of microtubule motors during intracellular transport. Intracellular transport of cellular cargo is a fundamental process underlying cell growth and differentiation. Malfunctions associated with mutations in the transport machinery give rise to many developmental and neurological diseases. To understand how the microtubule motors, cytoplasmic dynein and kinesins, find and transport cargoes along microtubule tracks to specific cellular destinations, we need to know more about how motors are attached to, and released from, cargoes, how tracks are specified and maintained, and how the activity of cargo motor ensembles is coordinated in space and time. In this grant period, we will capitalize on the well-characterized motor neurons of Drosophila and the sophisticated genetic tool box available, to query the intact organism for insights into how these highly conserved motors and cellular transport mechanisms are coordinated.
In Aim 1 we are analyzing Spinocerebellar Ataxia Type 5 (SCA5) mutations linked to neurological disease to understand how spectrin functions in intracellular transport. We have found that mutations in ?-spectrin impair synaptic vesicle and organelle motility in neurons and disrupt neuronal morphogenesis. We discovered that a SCA5 mutation in the actin-binding domain of ?-spectrin causes it to bind actin with a 1000-fold increased affinity. Subsequent structural studies predict a similarly elevated affinity between ?-spectrin and the Arp1 filament of dynactin. We will determine if the loss of dynamic binding between ?-spectrin on the surface of a vesicle and the Arp1 subunit of the dynactin motor adapter, underlies SCA5-induced synaptic vesicle motility defects.
In Aims 2 and 3, we are identifying novel genes and pathways that regulate synaptic function using transport assays in motor neurons. We recently showed that a protein complex, Striatin-interacting phosphatase and kinase (STRIPAK), regulates axonal transport, and is in a complex with dynein. We will study how STRIPAK components mediate activation of axonal transport. In addition, we have uncovered evidence of trans-synaptic regulation of axonal transport involving the postsynaptic cytoskeleton. We will investigate the signaling mechanisms involved in the regulation of synaptic homeostasis by the postsynaptic cytoskeleton. Our studies will provide new insights in the mechanisms that regulate transport and potential avenues for the treatment of neurological diseases.

Public Health Relevance

My laboratory is applying genetic, molecular and biochemical approaches in Drosophila to study the molecular regulation of the cytoskeleton and associated motor proteins underlying intracellular transport. In particular, the extended morphology of axons and dendrites makes post-mitotic neurons especially dependent on polarized transport, and ideal for studying the regulation of transport. The perturbation of axonal transport may represent a common pathogenic mechanism in neurodegenerative diseases including Huntington's disease, Parkinson's disease, Alzheimer's disease and Amyotrophic Lateral Sclerosis, and Spinocerebellar Ataxias.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM044757-26
Application #
10098324
Study Section
Nuclear and Cytoplasmic Structure/Function and Dynamics Study Section (NCSD)
Program Officer
Ainsztein, Alexandra M
Project Start
1990-07-01
Project End
2023-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
26
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Genetics
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
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Bhaban, Shreyas; Talukdar, Saurav; Li, Mingang et al. (2018) Single Molecule Studies Enabled by Model-Based Controller Design. IEEE ASME Trans Mechatron 23:1532-1542
Neisch, Amanda L; Neufeld, Thomas P; Hays, Thomas S (2017) A STRIPAK complex mediates axonal transport of autophagosomes and dense core vesicles through PP2A regulation. J Cell Biol 216:441-461
Avery, Adam W; Fealey, Michael E; Wang, Fengbin et al. (2017) Structural basis for high-affinity actin binding revealed by a ?-III-spectrin SCA5 missense mutation. Nat Commun 8:1350
Avery, Adam W; Thomas, David D; Hays, Thomas S (2017) ?-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization. Proc Natl Acad Sci U S A 114:E9376-E9385
Neisch, Amanda L; Avery, Adam W; Machamer, James B et al. (2016) Methods to identify and analyze gene products involved in neuronal intracellular transport using Drosophila. Methods Cell Biol 131:277-309
Mauvezin, Caroline; Neisch, Amanda L; Ayala, Carlos I et al. (2016) Coordination of autophagosome-lysosome fusion and transport by a Klp98A-Rab14 complex in Drosophila. J Cell Sci 129:971-82
Bhaban, Shreyas; Materassi, Donatello; Li, Mingang et al. (2016) Interrogating Emergent Transport Properties for Molecular Motor Ensembles: A Semi-analytical Approach. PLoS Comput Biol 12:e1005152
McIntosh, J Richard; Hays, Thomas (2016) A Brief History of Research on Mitotic Mechanisms. Biology (Basel) 5:
Avery, Adam W; Crain, Jonathan; Thomas, David D et al. (2016) A human ?-III-spectrin spinocerebellar ataxia type 5 mutation causes high-affinity F-actin binding. Sci Rep 6:21375

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