The objective of this research is to obtain accurate structures of lipid bilayers, especially regarding the area per molecule and the thickness of the hydrophobic layer. Such structures will provide a data base for evaluating how different lipids affect biomembrane function, including passive transport and protein-lipid interaction and, also, for evaluating the various interaction energies that determine the structure of membranes. The research utilizes a combination of diffraction and volumetric data. The volumetric data is being obtained using the neutral flotation technique. The diffraction data is being obtained using a rotating anode source with a variety of detectors, including a high precision scintillator-photomultiplier detector, a linear position sensitive detector and film. The use of new electron density models allows the incorporation of both low angle and wide angle x-ray and neutron diffraction data and the volumetric data into the same analysis. New quantitative mathematical relations connect the information perpendicular to the bilayer to information lateral to the bilayer. X-ray diffraction measurements will be made on fully hydrated unoriented multilamellar liposomes, oriented multilamellar liposomes, and large and small unilamellar vesicles to test hypotheses regarding the effect of interlamellar interactions on the structure of lipid bilayers. Determination of the structure of the biologically relevant chain disordered phases will be aided by bootstrapping from the determination of the structure of the chain ordered phases that occur at lower temperature.
| Nagle, John F; Jablin, Michael S; Tristram-Nagle, Stephanie (2016) Sugar does not affect the bending and tilt moduli of simple lipid bilayers. Chem Phys Lipids 196:76-80 |
| Stetten, Amy Z; Moraca, Grace; Corcoran, Timothy E et al. (2016) Enabling Marangoni flow at air-liquid interfaces through deposition of aerosolized lipid dispersions. J Colloid Interface Sci 484:270-278 |
| Nagle, John F; Akabori, Kiyotaka; Treece, Bradley W et al. (2016) Determination of mosaicity in oriented stacks of lipid bilayers. Soft Matter 12:1884-91 |
| O'Neil, Lauren; Andenoro, Kathryn; Pagano, Isabella et al. (2016) HIV-1 matrix-31 membrane binding peptide interacts differently with membranes containing PS vs. PI(4,5)P2. Biochim Biophys Acta 1858:3071-3081 |
| Reese, Caleb W; Strango, Zachariah I; Dell, Zachary R et al. (2015) Structural insights into the cubic-hexagonal phase transition kinetics of monoolein modulated by sucrose solutions. Phys Chem Chem Phys 17:9194-204 |
| Neale, Chris; Huang, Kun; García, Angel E et al. (2015) Penetration of HIV-1 Tat47-57 into PC/PE Bilayers Assessed by MD Simulation and X-ray Scattering. Membranes (Basel) 5:473-94 |
| Nagle, John F; Jablin, Michael S; Tristram-Nagle, Stephanie et al. (2015) What are the true values of the bending modulus of simple lipid bilayers? Chem Phys Lipids 185:3-10 |
| Kollmitzer, Benjamin; Heftberger, Peter; Podgornik, Rudolf et al. (2015) Bending Rigidities and Interdomain Forces in Membranes with Coexisting Lipid Domains. Biophys J 108:2833-42 |
| Akabori, Kiyotaka; Nagle, John F (2015) Structure of the DMPC lipid bilayer ripple phase. Soft Matter 11:918-26 |
| Ma, Yicong; Ghosh, Sajal K; Bera, Sambhunath et al. (2015) Accurate calibration and control of relative humidity close to 100% by X-raying a DOPC multilayer. Phys Chem Chem Phys 17:3570-6 |
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