A major goal of embryogenesis is the establishment of body form. To accomplish this, each cell must differentiate appropriately for its position. Several questions about """"""""positional information"""""""" arise. How is it generated? Of what is it constituted? How is it read accurately by each cell? And, once read, how do cells execute commands to differentiate properly? For all their complexity, these issues have become tractable as a consequence of molecular genetic analysis of embryogenesis in the fruit fly. This proposal addresses the nature of positional information and how specific cells read that information. At the cellular blastoderm in embryogenesis, pair-rule proteins cooperate to activate the expression of identity-determining genes in specific cells. In this manner some cells are given their positional identities directly. The genes specifying identity are members of the segment polarity gene class. Subsequently, other cells have their identities established by virtue of cell communication circuits that are controlled by the segment polarity genes. The pair-rule gene oddpaired plays a central role in activating three segment polarity genes, wingless, engrailed and gooseberry, each of which has distinct roles in cell patterning. This proposal addresses the mechanism by which oddpaired activates these cell patterning genes. The mechanisms revealed by these studies are likely to be at work in other organisms where cellular interactions and inductive events predominate during development.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM045747-03
Application #
2183367
Study Section
Genetics Study Section (GEN)
Project Start
1992-01-01
Project End
1995-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Anatomy/Cell Biology
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Ly, Dan; Resch, Erin; Ordiway, George et al. (2017) Asymmetrically deployed actomyosin-based contractility generates a boundary between developing leg segments in Drosophila. Dev Biol 429:165-176
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Donoughe, Seth; DiNardo, Stephen (2011) dachsous and frizzled contribute separately to planar polarity in the Drosophila ventral epidermis. Development 138:2751-9
Simone, Robert P; DiNardo, Stephen (2010) Actomyosin contractility and Discs large contribute to junctional conversion in guiding cell alignment within the Drosophila embryonic epithelium. Development 137:1385-94
Dilks, Stacie A; DiNardo, Stephen (2010) Non-cell-autonomous control of denticle diversity in the Drosophila embryo. Development 137:1395-404
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Walters, James W; Dilks, Stacie A; DiNardo, Stephen (2006) Planar polarization of the denticle field in the Drosophila embryo: roles for Myosin II (zipper) and fringe. Dev Biol 297:323-39
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Walters, James W; Munoz, Claudia; Paaby, Annalise B et al. (2005) Serrate-Notch signaling defines the scope of the initial denticle field by modulating EGFR activation. Dev Biol 286:415-26

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