We will study the molecular structure of tubulin in crystalline sheets which are induced by small concentrations of zinc ions. Structural information obtained should help in understanding the ways in which tubulin in microtubules and other assemblies carries out its myriad of functions during the development and life of a cell. Electron crystallography, which is ideally suited to the study of twodimensional specimens such as these sheets, will be the main experimental technique. Data from electron micrographs of the crystalline lattice and, where possible, electron diffraction patterns can be combined to yield a three-dimensional density map of the molecule, and this map can then be interpreted in terms of the atomic structure. Electron crystallographic techniques have developed over the last few years to the point where we can make significant contributions using the types of tubulin sheets which have been used in previously published structural studies. We will, though, spend some effort on improving the size and quality of these sheets in order to make extension of this work to high resolution simpler and more efficient.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM046033-01A1
Application #
3305509
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1992-08-01
Project End
1996-07-31
Budget Start
1992-08-01
Budget End
1993-07-31
Support Year
1
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Lawrence Berkeley National Laboratory
Department
Type
Organized Research Units
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720
Sindelar, Charles V; Downing, Kenneth H (2010) An atomic-level mechanism for activation of the kinesin molecular motors. Proc Natl Acad Sci U S A 107:4111-6
Cochran, Jared C; Sindelar, Charles V; Mulko, Natasha K et al. (2009) ATPase cycle of the nonmotile kinesin NOD allows microtubule end tracking and drives chromosome movement. Cell 136:110-22
Dietrich, Kristen A; Sindelar, Charles V; Brewer, Paul D et al. (2008) The kinesin-1 motor protein is regulated by a direct interaction of its head and tail. Proc Natl Acad Sci U S A 105:8938-43
Wolf, S G; Mosser, G; Downing, K H (1993) Tubulin conformation in zinc-induced sheets and macrotubes. J Struct Biol 111:190-9