The aim of this application is to characterize the molecular events involved in the assembly of gap junction channels. The proposal is divided into three aims. First, the investigators will characterize the mechanisms of post-translational control of connexin transport to the plasma membrane. Second, they will analyze the regulation of Cx43 hemichannel gating and the lateral migration of hemichannels. Third, they will study the mechanism(s) by which phosphorylation of Cx43 leads to an inhibition of Gj assembly and communication.
Johnson, Ross G; Le, Hung C; Evenson, Kristen et al. (2016) Connexin Hemichannels: Methods for Dye Uptake and Leakage. J Membr Biol 249:713-741 |
Johnson, Ross G; Reynhout, James K; TenBroek, Erica M et al. (2012) Gap junction assembly: roles for the formation plaque and regulation by the C-terminus of connexin43. Mol Biol Cell 23:71-86 |
Essner, J J; Laing, J G; Beyer, E C et al. (1996) Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos. Dev Biol 177:449-62 |