Abstract

The genes for Histidine UTilization (hut) in Klebsiella aerogenes are typical of a large number of genes whose expression is strictly regulated by the available supply of nitrogen. Thus nitrogen regulation of hut serves as a model system for this kind of pleiotropic control. The goal of the research proposed here is to develop an in vitro transcription system for the hut operon of K. aerogenes that is responsive to regulation by the nitrogen regulatory factor(s) and to study the molecular basis for that regulation. The specific objectives of the five-year proposal period are: (1) To determine the mechanism of carbon regulation at the promoter of the hutUH operon, (2) To begin an analysis of the nitrogen regulation at the promoter of the hutUH operon, and (3) To extend our understanding of the hut operons themselves.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047156-13
Application #
3306641
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1979-05-01
Project End
1995-06-30
Budget Start
1992-07-01
Budget End
1993-06-30
Support Year
13
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
Schools of Arts and Sciences
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Bender, Robert A (2010) A NAC for regulating metabolism: the nitrogen assimilation control protein (NAC) from Klebsiella pneumoniae. J Bacteriol 192:4801-11
Frisch, Ryan L; Bender, Robert A (2010) Expanded role for the nitrogen assimilation control protein in the response of Klebsiella pneumoniae to nitrogen stress. J Bacteriol 192:4812-20
Rosario, Christopher J; Frisch, Ryan L; Bender, Robert A (2010) The LysR-type nitrogen assimilation control protein forms complexes with both long and short DNA binding sites in the absence of coeffectors. J Bacteriol 192:4827-33
Frisch, Ryan L; Bender, Robert A (2010) Properties of the NAC (nitrogen assimilation control protein)-binding site within the ureD promoter of Klebsiella pneumoniae. J Bacteriol 192:4821-6
Rosario, Christopher J; Janes, Brian K; Bender, Robert A (2010) Genetic analysis of the nitrogen assimilation control protein from Klebsiella pneumoniae. J Bacteriol 192:4834-46
Goss, Thomas J (2008) The ArgP protein stimulates the Klebsiella pneumoniae gdhA promoter in a lysine-sensitive manner. J Bacteriol 190:4351-9
de la Riva, Lucia; Badia, Josefa; Aguilar, Juan et al. (2008) The hpx genetic system for hypoxanthine assimilation as a nitrogen source in Klebsiella pneumoniae: gene organization and transcriptional regulation. J Bacteriol 190:7892-903
Liu, Qiong; Bender, Robert A (2007) Complex regulation of urease formation from the two promoters of the ure operon of Klebsiella pneumoniae. J Bacteriol 189:7593-9
Rosario, Christopher J; Bender, Robert A (2005) Importance of tetramer formation by the nitrogen assimilation control protein for strong repression of glutamate dehydrogenase formation in Klebsiella pneumoniae. J Bacteriol 187:8291-9
Shankar-Sinha, Sunita; Valencia, Gabriel A; Janes, Brian K et al. (2004) The Klebsiella pneumoniae O antigen contributes to bacteremia and lethality during murine pneumonia. Infect Immun 72:1423-30

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