Abstract

The eukaryotic cell cycle is central to proliferation, development, and many health issues including cancer. The budding yeast is a well-established model system for studying evolutionarily conserved aspects of cell cycle control. The present application seeks to use recently developed proteomic tools to identify and examine the roles of cyclin-associated proteins in cell cycle control. Additional related experiments on the fundamental nature of the cyclin-dependent kinase/APC proteolysis oscillator are proposed. This oscillator is highly conserved and essential for cell cycle control throughout the eukaryotes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM047238-09
Application #
6572052
Study Section
Genetics Study Section (GEN)
Program Officer
Zatz, Marion M
Project Start
1993-05-01
Project End
2006-11-30
Budget Start
2002-12-05
Budget End
2003-11-30
Support Year
9
Fiscal Year
2003
Total Cost
$325,650
Indirect Cost

  Institution

Name
Rockefeller University
Department
Genetics
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Li, Yinyin; Cross, Frederick R; Chait, Brian T (2014) Method for identifying phosphorylated substrates of specific cyclin/cyclin-dependent kinase complexes. Proc Natl Acad Sci U S A 111:11323-8
Cross, Frederick R; Pecani, Kresti (2011) Efficient and rapid exact gene replacement without selection. Yeast 28:167-79
Rosenberg, Jessica S; Cross, Frederick R; Funabiki, Hironori (2011) KNL1/Spc105 recruits PP1 to silence the spindle assembly checkpoint. Curr Biol 21:942-7
Cross, Frederick R; Buchler, Nicolas E; Skotheim, Jan M (2011) Evolution of networks and sequences in eukaryotic cell cycle control. Philos Trans R Soc Lond B Biol Sci 366:3532-44
Oikonomou, Catherine; Cross, Frederick R (2011) Rising cyclin-CDK levels order cell cycle events. PLoS One 6:e20788
Wäsch, R; Robbins, J A; Cross, F R (2010) The emerging role of APC/CCdh1 in controlling differentiation, genomic stability and tumor suppression. Oncogene 29:1-10
Oikonomou, Catherine; Cross, Frederick R (2010) Frequency control of cell cycle oscillators. Curr Opin Genet Dev 20:605-12
Robbins, Jonathan A; Cross, Frederick R (2010) Requirements and reasons for effective inhibition of the anaphase promoting complex activator CDH1. Mol Biol Cell 21:914-25
Lu, Ying; Cross, Frederick R (2010) Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator. Cell 141:268-79
Di Talia, Stefano; Wang, Hongyin; Skotheim, Jan M et al. (2009) Daughter-specific transcription factors regulate cell size control in budding yeast. PLoS Biol 7:e1000221

Showing the most recent 10 out of 44 publications