Abstract

The long-term goal is to understand the function of actin in yeast. In the present application, Dr. Cooper proposes to investigate how how actin assembles into filaments and larger supramolecular structures and how one of these structures, """"""""actin patches"""""""" moves about the cell and forms clusters. A variety of biochemical, cell biological and genetic approaches are described to identify motors or accessory proteins necessary for patch motility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047337-07
Application #
2857149
Study Section
Special Emphasis Panel (ZRG2-BIOL-2 (01))
Project Start
1993-01-01
Project End
2000-12-31
Budget Start
1999-01-01
Budget End
1999-12-31
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Physiology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Aiken, Jayne; Sept, David; Costanzo, Michael et al. (2014) Genome-wide analysis reveals novel and discrete functions for tubulin carboxy-terminal tails. Curr Biol 24:1295-1303
Siglin, Amanda E; Sun, Shangjin; Moore, Jeffrey K et al. (2013) Dynein and dynactin leverage their bivalent character to form a high-affinity interaction. PLoS One 8:e59453
Nelson, Scott A; Sanson, Anthony M; Park, Hay-Oak et al. (2012) A novel role for the GTPase-activating protein Bud2 in the spindle position checkpoint. PLoS One 7:e36127
Stuchell-Brereton, Melissa D; Siglin, Amanda; Li, Jun et al. (2011) Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning. Mol Biol Cell 22:2690-701
Moore, Jeffrey K; Chudalayandi, Prakash; Heil-Chapdelaine, Richard A et al. (2010) The spindle position checkpoint is coordinated by the Elm1 kinase. J Cell Biol 191:493-503
Moore, Jeffrey K; Cooper, John A (2010) Coordinating mitosis with cell polarity: Molecular motors at the cell cortex. Semin Cell Dev Biol 21:283-9
Galletta, Brian J; Cooper, John A (2009) Actin and endocytosis: mechanisms and phylogeny. Curr Opin Cell Biol 21:20-7
Pollard, Thomas D; Cooper, John A (2009) Actin, a central player in cell shape and movement. Science 326:1208-12
Moore, Jeffrey K; Magidson, Valentin; Khodjakov, Alexey et al. (2009) The spindle position checkpoint requires positional feedback from cytoplasmic microtubules. Curr Biol 19:2026-30
Moore, Jeffrey K; Sept, David; Cooper, John A (2009) Neurodegeneration mutations in dynactin impair dynein-dependent nuclear migration. Proc Natl Acad Sci U S A 106:5147-52

Showing the most recent 10 out of 48 publications