Abstract

This project is designed to fuse the expertise of laboratories at two different institutions in order to contribute man's understanding of cell growth and development. These processes define the architecture and health of all eucaryotic organisms from man to higher plants. Plant cells, however, provide a simplified model system for all eucaryotic growth and development, one that can now be chemically and genetically manipulated, even to the point of transgenic organisms. Specifically, the enzymes controlling the biosynthesis of a unique growth factor, the dehydrodiconiferyl alcohol glucosides (DCG), will be isolated, characterized, and their respective genes cloned. Various chemical and genetic approaches will be employed to manipulate the production of these growth factors in vivo manipulating the levels and activities of the enzymes. The manipulation of one pathway should simplify the other interacting systems that are certainly involved in the control of cell growth. The definition of the pathway involved in DCG production and the control of the biocatalysts involved should provide the first step in understanding of one of the black boxes of biochemistry-how information is received and processed in the control of cell growth.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047369-03
Application #
2184772
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1992-04-01
Project End
1996-03-31
Budget Start
1994-04-01
Budget End
1996-03-31
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Nair, Gauri R; Lai, Xiaoqin; Wise, Arlene A et al. (2011) The integrity of the periplasmic domain of the VirA sensor kinase is critical for optimal coordination of the virulence signal response in Agrobacterium tumefaciens. J Bacteriol 193:1436-48
Wise, Arlene A; Fang, Fang; Lin, Yi-Han et al. (2010) The receiver domain of hybrid histidine kinase VirA: an enhancing factor for vir gene expression in Agrobacterium tumefaciens. J Bacteriol 192:1534-42
He, Fanglian; Nair, Gauri R; Soto, Cinque S et al. (2009) Molecular basis of ChvE function in sugar binding, sugar utilization, and virulence in Agrobacterium tumefaciens. J Bacteriol 191:5802-13
Gao, Rong; Lynn, David G (2007) Integration of rotation and piston motions in coiled-coil signal transduction. J Bacteriol 189:6048-56
Yuan, Ze-Chun; Edlind, Merritt P; Liu, Pu et al. (2007) The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium. Proc Natl Acad Sci U S A 104:11790-5
Maresh, Justin; Zhang, Jin; Tzeng, Yih-Ling et al. (2007) Rational design of inhibitors of VirA-VirG two-component signal transduction. Bioorg Med Chem Lett 17:3281-6
Wise, Arlene A; Liu, Zhenying; Binns, Andrew N (2006) Three methods for the introduction of foreign DNA into Agrobacterium. Methods Mol Biol 343:43-53
Maresh, Justin; Zhang, Jin; Lynn, David G (2006) The innate immunity of maize and the dynamic chemical strategies regulating two-component signal transduction in Agrobacterium tumefaciens. ACS Chem Biol 1:165-75
Wise, Arlene A; Liu, Zhenying; Binns, Andrew N (2006) Nucleic acid extraction from Agrobacterium strains. Methods Mol Biol 343:67-76
Wise, Arlene A; Liu, Zhenying; Binns, Andrew N (2006) Culture and maintenance of Agrobacterium strains. Methods Mol Biol 343:3-13

Showing the most recent 10 out of 22 publications