Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM047399-20A2
Application #
6332285
Study Section
Biochemistry Study Section (BIO)
Program Officer
Jones, Warren
Project Start
1991-07-01
Project End
2005-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
20
Fiscal Year
2001
Total Cost
$255,603
Indirect Cost
Name
Wayne State University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202
Hervé, Guy; Evans, Hedeel Guy; Fernado, Roshini et al. (2017) Activation of Latent Dihydroorotase from Aquifex aeolicus by Pressure. J Biol Chem 292:629-637
Evans, Hedeel Guy; Fernando, Roshini; Vaishnav, Asmita et al. (2014) Intersubunit communication in the dihydroorotase-aspartate transcarbamoylase complex of Aquifex aeolicus. Protein Sci 23:100-9
Edwards, Brian F P; Fernando, Roshini; Martin, Philip D et al. (2013) The mononuclear metal center of type-I dihydroorotase from Aquifex aeolicus. BMC Biochem 14:36
Zhang, Pengfei; Martin, Philip D; Purcarea, Cristina et al. (2009) Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis. Biochemistry 48:766-78
Serre, V; Guy, H; Liu, X et al. (1998) Allosteric regulation and substrate channeling in multifunctional pyrimidine biosynthetic complexes: analysis of isolated domains and yeast-mammalian chimeric proteins. J Mol Biol 281:363-77
Liu, X; Guy, H I; Evans, D R (1994) Identification of the regulatory domain of the mammalian multifunctional protein CAD by the construction of an Escherichia coli hamster hybrid carbamyl-phosphate synthetase. J Biol Chem 269:27747-55
Guy, H I; Evans, D R (1994) Cloning and expression of the mammalian multifunctional protein CAD in Escherichia coli. Characterization of the recombinant protein and a deletion mutant lacking the major interdomain linker. J Biol Chem 269:23808-16
Guy, H I; Evans, D R (1994) Function of the polypeptide chain segment connecting the dihydroorotase and aspartate transcarbamylase domains in the mammalian multifunctional CAD. Adv Exp Med Biol 370:729-33
Zimmermann, B H; Evans, D R (1993) Cloning, overexpression, and characterization of the functional dihydroorotase domain of the mammalian multifunctional protein CAD. Biochemistry 32:1519-27
Bergh, S T; Evans, D R (1993) Subunit structure of a class A aspartate transcarbamoylase from Pseudomonas fluorescens. Proc Natl Acad Sci U S A 90:9818-22

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