Abstract

This research is directed toward understanding biological assembly processes at the molecular level, using assembly of the bacteriophage lambda virion as a model. The research focuses on assembly processes in which one protein influences or directs the assembly of another, and seeks to understand the detailed mechanisms behind such events. Specific problems being addressed include the structure and regulation of the E. coli GroE proteins, which are host proteins required for correct virus assembly, the nature and role of protein processing during virus assembly, and the mechanisms by which the tail tape measure protein determines tail length.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
9R01GM047795-17
Application #
3307192
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1977-12-01
Project End
1995-08-31
Budget Start
1991-09-30
Budget End
1992-08-31
Support Year
17
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
Schools of Arts and Sciences
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Hua, Jianfei; Huet, Alexis; Lopez, Carlos A et al. (2017) Capsids and Genomes of Jumbo-Sized Bacteriophages Reveal the Evolutionary Reach of the HK97 Fold. MBio 8:
Tso, DanJu; Peebles, Craig L; Maurer, Joshua B et al. (2017) On the catalytic mechanism of bacteriophage HK97 capsid crosslinking. Virology 506:84-91
Hasek, Mary L; Maurer, Joshua B; Hendrix, Roger W et al. (2017) Flexible Connectors between Capsomer Subunits that Regulate Capsid Assembly. J Mol Biol 429:2474-2489
Huet, Alexis; Duda, Robert L; Hendrix, Roger W et al. (2016) Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex. J Mol Biol 428:165-181
Casjens, Sherwood R; Hendrix, Roger W (2015) Bacteriophage lambda: Early pioneer and still relevant. Virology 479-480:310-30
Cardone, Giovanni; Duda, Robert L; Cheng, Naiqian et al. (2014) Metastable intermediates as stepping stones on the maturation pathways of viral capsids. MBio 5:e02067
Pope, Welkin H; Jacobs-Sera, Deborah; Russell, Daniel A et al. (2014) Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood. MBio 5:e02145
Xu, Jun; Hendrix, Roger W; Duda, Robert L (2014) Chaperone-protein interactions that mediate assembly of the bacteriophage lambda tail to the correct length. J Mol Biol 426:1004-18
Tso, Dan-ju; Hendrix, Roger W; Duda, Robert L (2014) Transient contacts on the exterior of the HK97 procapsid that are essential for capsid assembly. J Mol Biol 426:2112-29
Oh, Bonnie; Moyer, Crystal L; Hendrix, Roger W et al. (2014) The delta domain of the HK97 major capsid protein is essential for assembly. Virology 456-457:171-8

Showing the most recent 10 out of 36 publications