Abstract

The discovery of two novel yeast proteins, Duo282p, an essential spindle component, and Jsn1p, a protein that appears to influence microtubule dynamics and is enriched on microtubule affinity columns, provides unique opportunities to determine how accessory proteins regulate the dynamics of microtubule assembly in vivo. In addition, a third protein has been discovered which appears to provide a functional link between the microtubule and actin cytoskeletons during mitosis. This protein, Cor1p apparently serves as a link between microtubules and the cortical actin cytoskeleton during spindle orientation. The focus of this proposal is to investigate the mechanisms by which these novel proteins influence microtubule dynamics and spindle function.
The specific aims are to: (1) elucidate the nature and function of Cor1p's interaction with the actin and microtubule cytoskeletons and to genetically and biochemically identify proteins which interact with yeast Cor1p; (2) determine the specific function of yeast Duo282p in spindle function and assembly and determine the nature of its interaction with spindles; (3) elucidate the role of Jsn1p in regulating microtuble dynamics by identifying proteins which function with or in parallel with Jsn1p. In total, these studies will define components of the microtubule and actin cytoskeletons that are required to ensure proper chromosome inheritance.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047842-05
Application #
2872676
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1992-09-30
Project End
2002-01-31
Budget Start
1999-02-01
Budget End
2000-01-31
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Ibarlucea-Benitez, Itziar; Ferro, Luke S; Drubin, David G et al. (2018) Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly. J Cell Biol 217:1687-1700
Peng, Yutian; Grassart, Alexandre; Lu, Rebecca et al. (2015) Casein kinase 1 promotes initiation of clathrin-mediated endocytosis. Dev Cell 32:231-40
Peng, Yutian; Moritz, Michelle; Han, Xuemei et al. (2015) Interaction of CK1? with ?TuSC ensures proper microtubule assembly and spindle positioning. Mol Biol Cell 26:2505-18
Krefman, Nathaniel I; Drubin, David G; Barnes, Georjana (2015) Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment. Mol Biol Cell 26:2620-39
Pigula, Adrianne; Drubin, David G; Barnes, Georjana (2014) Regulation of mitotic spindle disassembly by an environmental stress-sensing pathway in budding yeast. Genetics 198:1043-57
Cormier, Anthony; Drubin, David G; Barnes, Georjana (2013) Phosphorylation regulates kinase and microtubule binding activities of the budding yeast chromosomal passenger complex in vitro. J Biol Chem 288:23203-11
Faust, Ann Marie E; Wong, Catherine C L; Yates 3rd, John R et al. (2013) The FEAR protein Slk19 restricts Cdc14 phosphatase to the nucleus until the end of anaphase, regulating its participation in mitotic exit in Saccharomyces cerevisiae. PLoS One 8:e73194
Woodruff, Jeffrey B; Drubin, David G; Barnes, Georjana (2012) Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle. Mol Biol Cell 23:258-67
Ramey, Vincent H; Wang, Hong-Wei; Nakajima, Yuko et al. (2011) The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule. Mol Biol Cell 22:457-66
Peng, Yutian; Wong, Catherine C L; Nakajima, Yuko et al. (2011) Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation. Mol Biol Cell 22:2680-9

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