Abstract

This project is entirely devoted to the isolation and characterization of RNA catalysts which activate animo acids (by acyl phosphoanhydride formation), transfer amino acids to ester linkage on the 3' terminus of RNA, and form peptide bonds. That is, we are seeking RNA catalysts that carry out the three major biochemical reactions of genetic translation. Because RNA catalysis seems likely to be recognized as responsible for essential cellular reactions, and these catalysts will all he novel, these studies may serve as the foundation for definition of diseases and dysfunctions of RNA catalysis, just as biochemical study of protein catalysts has resulted in the definition of many diseases traceable to defective protein catalysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM048080-05
Application #
2415176
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1993-05-01
Project End
2000-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Colorado at Boulder
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309

  Publications

Yarus, Michael (2017) The Genetic Code and RNA-Amino Acid Affinities. Life (Basel) 7:
Turk-Macleod, Rebecca M; Puthenvedu, Deepa; Majerfeld, Irene et al. (2012) The plausibility of RNA-templated peptides: simultaneous RNA affinity for adjacent peptide side chains. J Mol Evol 74:217-25
Turk, Rebecca M; Illangasekare, Mali; Yarus, Michael (2011) Catalyzed and spontaneous reactions on ribozyme ribose. J Am Chem Soc 133:6044-50
Yarus, Michael (2011) Getting past the RNA world: the initial Darwinian ancestor. Cold Spring Harb Perspect Biol 3:
Illangasekare, Mali; Turk, Rebecca; Peterson, G Colin et al. (2010) Chiral histidine selection by D-ribose RNA. RNA 16:2370-83
Majerfeld, Irene; Chocholousova, Jana; Malaiya, Vikas et al. (2010) Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA. RNA 16:1915-24
Turk, Rebecca M; Chumachenko, Nataliya V; Yarus, Michael (2010) Multiple translational products from a five-nucleotide ribozyme. Proc Natl Acad Sci U S A 107:4585-9
Kennedy, Ryan; Lladser, Manuel E; Wu, Zhiyuan et al. (2010) Natural and artificial RNAs occupy the same restricted region of sequence space. RNA 16:280-9
Janas, Teresa; Widmann, Jeremy Joseph; Knight, Rob et al. (2010) Simple, recurring RNA binding sites for L-arginine. RNA 16:805-16
Chumachenko, N V; Novikov, Y; Yarus, M (2009) Rapid and simple ribozymic aminoacylation using three conserved nucleotides. J Am Chem Soc 131:5257-63

Showing the most recent 10 out of 34 publications