Abstract

The goals of specific aim 1 (project A) are to carefully state the intermediates in nuclear pore assembly (using chemical staging reagents such as wheat germ agglutinin, BAPTA, and low temperature) and to identify soluble factors that promote pore formation and stabilization. The goal of specific aim 2 (project B) is to test the roles of the membrane-embedded nucleoporins, gp120 and pom 121, in the membrane fusion' event that is thought to generate the pore. The goal of specific aim 3 (project C) is to identify and analyze soluble nucleoporins that associate in a functionally significant manner with gp120 and pom 121.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM048646-08S1
Application #
6584762
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Shapiro, Bert I
Project Start
1994-05-01
Project End
2003-04-30
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
8
Fiscal Year
2002
Total Cost
$97,057
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Berk, Jason M; Wilson, Katherine L (2016) Simple Separation of Functionally Distinct Populations of Lamin-Binding Proteins. Methods Enzymol 569:101-14
Mojica, Sergio A; Hovis, Kelley M; Frieman, Matthew B et al. (2015) SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors. Mol Biol Cell 26:1918-34
Wilson, Katherine L; Weis, Karsten (2015) Editorial overview: Cell nucleus: Nuclear structure and organization—open frontiers in cell and genome biology. Curr Opin Cell Biol 34:v-vi
Berk, Jason M; Simon, Dan N; Jenkins-Houk, Clifton R et al. (2014) The molecular basis of emerin-emerin and emerin-BAF interactions. J Cell Sci 127:3956-69
Bar, Daniel Z; Davidovich, Maya; Lamm, Ayelet T et al. (2014) BAF-1 mobility is regulated by environmental stresses. Mol Biol Cell 25:1127-36
Berk, Jason M; Tifft, Kathryn E; Wilson, Katherine L (2013) The nuclear envelope LEM-domain protein emerin. Nucleus 4:298-314
Simon, Dan N; Wilson, Katherine L (2013) Partners and post-translational modifications of nuclear lamins. Chromosoma 122:13-31
Berk, Jason M; Maitra, Sushmit; Dawdy, Andrew W et al. (2013) O-Linked ?-N-acetylglucosamine (O-GlcNAc) regulates emerin binding to barrier to autointegration factor (BAF) in a chromatin- and lamin B-enriched ""niche"". J Biol Chem 288:30192-209
Gjerstorff, Morten F; Rosner, Heike I; Pedersen, Christina B et al. (2012) GAGE cancer-germline antigens are recruited to the nuclear envelope by germ cell-less (GCL). PLoS One 7:e45819
Barkan, Rachel; Zahand, Adam J; Sharabi, Kfir et al. (2012) Ce-emerin and LEM-2: essential roles in Caenorhabditis elegans development, muscle function, and mitosis. Mol Biol Cell 23:543-52

Showing the most recent 10 out of 40 publications