The objective of the experiments proposed in this application are to identify and functionally characterize the structural proteins that comprise the spindle matrix through the following specific aims: l. Using a cell-free mitotic aster assembly system that is highly enriched in the components of the mitotic spindle matrix, the investigator will prepare antibodies that specifically recognize the three major proteins of the mitotic spindle matrix. 2. Using the antibodies prepared in Specific Aim l, the investigator will use immunodepletion and microinjection to determine if each of these proteins is required for the assembly of the mitotic spindle. 3. Using the antibodies prepared in Specific Aim l, he will isolate cDNA clones encoding each of these proteins of the mitotic spindle matrix and determine their primary amino acid sequence.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051542-04
Application #
6019026
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1996-08-01
Project End
2001-02-28
Budget Start
1999-08-01
Budget End
2001-02-28
Support Year
4
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Biochemistry
Type
Schools of Medicine
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
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