Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM052502-01A1
Application #
2191555
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1996-04-01
Project End
2000-03-31
Budget Start
1996-04-01
Budget End
1997-03-31
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Virginia
Department
Physiology
Type
Schools of Medicine
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Al-Shawi, Marwan K (2011) Catalytic and transport cycles of ABC exporters. Essays Biochem 50:63-83
Sekiya, Mizuki; Hosokawa, Hiroyuki; Nakanishi-Matsui, Mayumi et al. (2010) Single molecule behavior of inhibited and active states of Escherichia coli ATP synthase F1 rotation. J Biol Chem 285:42058-67
Sekiya, Mizuki; Nakamoto, Robert K; Al-Shawi, Marwan K et al. (2009) Temperature dependence of single molecule rotation of the Escherichia coli ATP synthase F1 sector reveals the importance of gamma-beta subunit interactions in the catalytic dwell. J Biol Chem 284:22401-10
Omote, Hiroshi; Al-Shawi, Marwan K (2006) Interaction of transported drugs with the lipid bilayer and P-glycoprotein through a solvation exchange mechanism. Biophys J 90:4046-59
Al-Shawi, Marwan K; Omote, Hiroshi (2005) The remarkable transport mechanism of P-glycoprotein: a multidrug transporter. J Bioenerg Biomembr 37:489-96
Omote, Hiroshi; Figler, Robert A; Polar, Mark K et al. (2004) Improved energy coupling of human P-glycoprotein by the glycine 185 to valine mutation. Biochemistry 43:3917-28
Al-Shawi, Marwan K; Polar, Mark K; Omote, Hiroshi et al. (2003) Transition state analysis of the coupling of drug transport to ATP hydrolysis by P-glycoprotein. J Biol Chem 278:52629-40
Omote, Hiroshi; Al-Shawi, Marwan K (2002) A novel electron paramagnetic resonance approach to determine the mechanism of drug transport by P-glycoprotein. J Biol Chem 277:45688-94
Figler, R A; Omote, H; Nakamoto, R K et al. (2000) Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purification of human P-glycoprotein. Arch Biochem Biophys 376:34-46
Nakamoto, R K; Ketchum, C J; Kuo, P H et al. (2000) Molecular mechanisms of rotational catalysis in the F(0)F(1) ATP synthase. Biochim Biophys Acta 1458:289-99

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