Alphaviruses and flaviviruses are the causative agents of severe human and animal illnesses such as encephalitis, polyarthritis, and dengue fever, with millions of cases in humans per year. These viruses also include potential bioterrorist agents such as the alphavirus Venezuelan Equine Encephalitis virus (a class B agent). Both alphaviruses and flaviviruses infect cells via a low pH-dependent membrane fusion reaction. Striking similarities exist between the native structures of the alphavirus and flavivirus membrane fusion proteins. Fusion by these """"""""class II"""""""" fusion proteins appears mechanistically quite different from that of the class I fusion proteins exemplified by influenza virus, in which the fusion protein forms a """"""""trimer of hairpins"""""""" with a central coiled-coil. The class II fusion proteins convert from a native dimer to a highly stable, non-coiled-coil based homotrimer. The goal of this application is to determine the molecular mechanism of membrane fusion of the alphavirus Semliki Forest virus (SFV), a member of the class II viruses and a highly developed system to study membrane fusion. Four key questions in the fusion mechanism of the class II proteins will be addressed: 1. Do class II fusion proteins refold to a """"""""hairpin"""""""" conformation in which the fusion peptide and transmembrane domains are at the same end of the molecule? 2. Does the SFV E1 fusion protein mediate fusion by the transition to the stable homotrimer or by posttrimer events? Class I fusion proteins harness the energy of the transition from their metastable native conformation to the stable final hairpin to carry out membrane fusion. Kinetic and inhibitor studies will be used to determine the mechanism by which the class II homotrimer mediates fusion. 3. What is the structure and mechanism of the E1 fusion peptide in the membrane? We will define the region of E1 that becomes membrane-bound at low pH and whether it inserts into the target or virus membranes. 4. What is the structural basis of the SFV E1 homotrimer? We will compare the properties of native E1 with the E1 homotrimer by biochemical, morphological, and structural analysis. Ultimately, molecular information on the class II fusion proteins will enable the design of specific antiviral therapies, and advance our general understanding of cellular and viral membrane fusion reactions.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM052929-11
Application #
6929782
Study Section
Virology Study Section (VR)
Program Officer
Basavappa, Ravi
Project Start
1995-08-15
Project End
2007-07-31
Budget Start
2005-08-01
Budget End
2006-07-31
Support Year
11
Fiscal Year
2005
Total Cost
$425,713
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Sánchez-San Martín, Claudia; Liu, Catherine Y; Kielian, Margaret (2009) Dealing with low pH: entry and exit of alphaviruses and flaviviruses. Trends Microbiol 17:514-21
Hewitt, F Curtis; Li, Chengwen; Gray, Steven J et al. (2009) Reducing the risk of adeno-associated virus (AAV) vector mobilization with AAV type 5 vectors. J Virol 83:3919-29
Kielian, Margaret (2006) Class II virus membrane fusion proteins. Virology 344:38-47
Liao, Maofu; Kielian, Margaret (2006) Functions of the stem region of the Semliki Forest virus fusion protein during virus fusion and assembly. J Virol 80:11362-9
Liao, Maofu; Kielian, Margaret (2006) Site-directed antibodies against the stem region reveal low pH-induced conformational changes of the Semliki Forest virus fusion protein. J Virol 80:9599-607
Liao, Maofu; Kielian, Margaret (2005) Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion. J Cell Biol 171:111-20
Zhang, Xinyong; Kielian, Margaret (2005) An interaction site of the envelope proteins of Semliki Forest virus that is preserved after proteolytic activation. Virology 337:344-52
Liao, Maofu; Kielian, Margaret (2005) The conserved glycine residues in the transmembrane domain of the Semliki Forest virus fusion protein are not required for assembly and fusion. Virology 332:430-7
Gibbons, Don L; Reilly, Brigid; Ahn, Anna et al. (2004) Purification and crystallization reveal two types of interactions of the fusion protein homotrimer of Semliki Forest virus. J Virol 78:3514-23
Gibbons, Don L; Ahn, Anna; Liao, Maofu et al. (2004) Multistep regulation of membrane insertion of the fusion peptide of Semliki Forest virus. J Virol 78:3312-8

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