This proposal outlines experiments to develop a better understanding of icosahedral RNA virus assembly. It focuses on specific interactions between capsid protein and viral RNA as well as aspects that control recognition, condensation and encapsidation of the viral genome. It addresses the unresolved issue of how multipartite RNA genomes are recognized and packaged into a single virion and it seeks to define the role of cellular parameters in this process. The experimental plan takes advantage of a highly developed model system represented by the icosahedral RNA containing nodaviruses. Nodaviruses are a family of genetically, biochemically and structurally exceptionally well characterized viruses that have proven to be highly tractable for the study of fundamental questions in virology, including particle assembly and packaging of the genome. The ? specific aims of this project are: (1) to investigate the role of subcellular compartmentalization in the ? Flock House Virus (FHV) life cycle using biochemical and genetic approaches; (2) to visualize ? cellular compartmentalization and spatial dynamics during FHV infection by fluorescence microscopy and electron microscopy; (3) to identify mechanistic strategies and molecular elements involved in packaging of the FHV genome and (4) to define the role of FHV coat protein in folding and condensation of viral RNA during assembly. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM053491-10
Application #
7017799
Study Section
Virology - A Study Section (VIRA)
Program Officer
Basavappa, Ravi
Project Start
1996-08-01
Project End
2009-03-31
Budget Start
2006-04-01
Budget End
2007-03-31
Support Year
10
Fiscal Year
2006
Total Cost
$408,446
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Short, James R; Speir, Jeffrey A; Gopal, Radhika et al. (2016) Role of Mitochondrial Membrane Spherules in Flock House Virus Replication. J Virol 90:3676-83
Gopal, Radhika; Venter, P Arno; Schneemann, Anette (2014) Differential segregation of nodaviral coat protein and RNA into progeny virions during mixed infection with FHV and NoV. Virology 454-455:280-90
Petrillo, Jessica E; Venter, P Arno; Short, James R et al. (2013) Cytoplasmic granule formation and translational inhibition of nodaviral RNAs in the absence of the double-stranded RNA binding protein B2. J Virol 87:13409-21
Liu, Bo; Behura, Susanta K; Clem, Rollie J et al. (2013) P53-mediated rapid induction of apoptosis conveys resistance to viral infection in Drosophila melanogaster. PLoS Pathog 9:e1003137
Jovel, Juan; Schneemann, Anette (2011) Molecular characterization of Drosophila cells persistently infected with Flock House virus. Virology 419:43-53
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Venter, P Arno; Marshall, Dawn; Schneemann, Anette (2009) Dual roles for an arginine-rich motif in specific genome recognition and localization of viral coat protein to RNA replication sites in flock house virus-infected cells. J Virol 83:2872-82
Venter, P A; Schneemann, A (2008) Recent insights into the biology and biomedical applications of Flock House virus. Cell Mol Life Sci 65:2675-87
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Lanman, Jason; Crum, John; Deerinck, Thomas J et al. (2008) Visualizing flock house virus infection in Drosophila cells with correlated fluorescence and electron microscopy. J Struct Biol 161:439-46

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