The diverse events of the eukaryotic cell cycle are induced and coordinated by the activity of cyclin-dependent kinases, which are activated at specific times during the cell cycle when they associate with members of the cyclin family of proteins. Although much has been learned about the regulation of cyclin-dependent kinase complexes, we still know virtually nothing about the pathways used by these kinases to induce cell cycle events. In simple organisms like budding yeast, a single cyclin-dependent kinase controls all of the events of the cell cycle. It is unknown how cyclin-dependent kinases attain such specificity. One possibility is that the different cyclins serve to determine the functions of specific cyclindependent kinase complexes. The long term goal of this project will be to gain a greater understanding of the pathways used by cyclin-dependent kinases to induce the events of the cell cycle. We will focus on the pathway used by cyclin-dependent kinases in budding yeast to control bud growth during mitosis, since this pathway is particularly amenable to detailed characterization, and preliminary work has already identified one of its components. A combination of genetics and biochemistry will be used to identify additional proteins that function to control bud growth during mitosis, and these will be studied as part of a detailed characterization of the pathway. The control of bud growth is likely to utilize fundamental mechanisms, since several of the proteins known to be involved are highly conserved between humans and yeast. An understanding of the pathways used to execute mitotic events is of considerable clinical relevance, since such pathways are likely to represent good targets for drugs designed to block the division of cancer cells.
| Alcaide-Gavilán, Maria; Lucena, Rafael; Schubert, Katherine A et al. (2018) Modulation of TORC2 Signaling by a Conserved Lkb1 Signaling Axis in Budding Yeast. Genetics 210:155-170 |
| Lucena, Rafael; Alcaide-Gavilán, Maria; Schubert, Katherine et al. (2018) Cell Size and Growth Rate Are Modulated by TORC2-Dependent Signals. Curr Biol 28:196-210.e4 |
| Vadia, Stephen; Tse, Jessica L; Lucena, Rafael et al. (2017) Fatty Acid Availability Sets Cell Envelope Capacity and Dictates Microbial Cell Size. Curr Biol 27:1757-1767.e5 |
| Leitao, Ricardo M; Kellogg, Douglas R (2017) The duration of mitosis and daughter cell size are modulated by nutrients in budding yeast. J Cell Biol 216:3463-3470 |
| Zapata, Jessica; Dephoure, Noah; Macdonough, Tracy et al. (2014) PP2ARts1 is a master regulator of pathways that control cell size. J Cell Biol 204:359-76 |
| Parnell, Emily J; Yu, Yaxin; Lucena, Rafael et al. (2014) The Rts1 regulatory subunit of PP2A phosphatase controls expression of the HO endonuclease via localization of the Ace2 transcription factor. J Biol Chem 289:35431-7 |
| McCusker, Derek; Royou, Anne; Velours, Christophe et al. (2012) Cdk1-dependent control of membrane-trafficking dynamics. Mol Biol Cell 23:3336-47 |
| McCusker, Derek; Kellogg, Douglas R (2012) Plasma membrane growth during the cell cycle: unsolved mysteries and recent progress. Curr Opin Cell Biol 24:845-51 |
| Egelhofer, Thea A; Villen, Judit; McCusker, Derek et al. (2008) The septins function in G1 pathways that influence the pattern of cell growth in budding yeast. PLoS One 3:e2022 |
| McCusker, Derek; Denison, Carilee; Anderson, Scott et al. (2007) Cdk1 coordinates cell-surface growth with the cell cycle. Nat Cell Biol 9:506-15 |
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