Abstract

This application is focused on the analysis of mechanisms and functions of MT-based motors that the investigators postulate drive specific transport event that occurs during early development. They propose to : 1) characterize the structure, function and domain organization of a putative minus-end-directed kinesin holoenzyme and to analyze its role in nuclear transport and mitosis; 2) to further analyze structure-function relationships in the morphogenesis and function of mitotic spindles; 3) to biochemically characterize the vesicular cargo of the sea urchin embryonic mitotic spindle for delivery to different destinations and 4) to purify the Drosophila Nod chromosomal kinesin-like motor from transgenic fly lines, in order to analyze the mechanism by which it transports chromosomes along MTs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM055507-04
Application #
6180676
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Deatherage, James F
Project Start
1997-07-01
Project End
2001-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
4
Fiscal Year
2000
Total Cost
$256,118
Indirect Cost

  Institution

Name
University of California Davis
Department
Anatomy/Cell Biology
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Scholey, Jonathan M; Civelekoglu-Scholey, Gul; Brust-Mascher, Ingrid (2016) Anaphase B. Biology (Basel) 5:
Brust-Mascher, Ingrid; Civelekoglu-Scholey, Gul; Scholey, Jonathan M (2015) Mechanism for Anaphase B: Evaluation of ""Slide-and-Cluster"" versus ""Slide-and-Flux-or-Elongate"" Models. Biophys J 108:2007-18
Scholey, Jessica E; Nithianantham, Stanley; Scholey, Jonathan M et al. (2014) Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers. Elife 3:e02217
Wang, Haifeng; Brust-Mascher, Ingrid; Civelekoglu-Scholey, Gul et al. (2013) Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation. J Cell Biol 203:35-46
Scholey, Jonathan M (2013) Compare and contrast the reaction coordinate diagrams for chemical reactions and cytoskeletal force generators. Mol Biol Cell 24:433-9
Acar, Seyda; Carlson, David B; Budamagunta, Madhu S et al. (2013) The bipolar assembly domain of the mitotic motor kinesin-5. Nat Commun 4:1343
de Lartigue, Jane; Brust-Mascher, Ingrid; Scholey, Jonathan M (2011) Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles. Cell Div 6:8
Wang, Haifeng; Brust-Mascher, Ingrid; Cheerambathur, Dhanya et al. (2010) Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion. Cytoskeleton (Hoboken) 67:715-28
Civelekoglu-Scholey, Gul; Tao, Li; Brust-Mascher, Ingrid et al. (2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope. J Cell Biol 188:49-68
Sommi, Patrizia; Ananthakrishnan, Revathi; Cheerambathur, Dhanya K et al. (2010) A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophila embryos. J Cell Sci 123:1862-72

Showing the most recent 10 out of 14 publications