Abstract

description): The long-term goal of this research is to better understand how circadian clocks work in eukaryotic cells, using Arabidopsis as a model system. Genetic approaches have revealed components of the circadian clock in Arabidopsis. Proposed studies will extend a mutant hunt using rhythmic bioluminescence in transgenic seedlings screened by high throughput automated monitoring of the circadian-regulated expression of CAB::luciferase construct. Several toc mutant genes, which are likely to encode central components of the Arabidopsis circadian system, will be cloned and characterized. Additionally, by crossing the luciferase fusion markers into available photoreceptor, phototransduction, and floral timing mutants, it is proposed to relate circadian function to previously defined pathways for photo-transduction and photoperiodic control of flowering. Ultimately, genes that are involved in many aspects of circadian timing will be cloned. Given the ubiquity of circadian-regulated physiology, the identification of common clock components may have an impact on understanding the mechanisms of circadian regulated functions in humans.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM056006-08
Application #
6650757
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Tompkins, Laurie
Project Start
1996-09-01
Project End
2004-08-31
Budget Start
2003-09-01
Budget End
2004-08-31
Support Year
8
Fiscal Year
2003
Total Cost
$348,283
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Kang, S Earl; Breton, Ghislain; Pruneda-Paz, Jose L (2018) Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens. Methods Mol Biol 1794:151-182
Li, Zheng; Bonaldi, Katia; Uribe, Francisco et al. (2018) A Localized Pseudomonas syringae Infection Triggers Systemic Clock Responses in Arabidopsis. Curr Biol 28:630-639.e4
Lee, Chin-Mei; Feke, Ann; Li, Man-Wah et al. (2018) Decoys Untangle Complicated Redundancy and Reveal Targets of Circadian Clock F-Box Proteins. Plant Physiol 177:1170-1186
Kubota, Akane; Ito, Shogo; Shim, Jae Sung et al. (2017) TCP4-dependent induction of CONSTANS transcription requires GIGANTEA in photoperiodic flowering in Arabidopsis. PLoS Genet 13:e1006856
Tripathi, Prateek; Carvallo, Marcela; Hamilton, Elizabeth E et al. (2017) Arabidopsis B-BOX32 interacts with CONSTANS-LIKE3 to regulate flowering. Proc Natl Acad Sci U S A 114:172-177
Tripathi, Prateek; Pruneda-Paz, José L; Kay, Steve A (2017) A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies. J Vis Exp :
Shani, Eilon; Salehin, Mohammad; Zhang, Yuqin et al. (2017) Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors. Curr Biol 27:437-444
Breton, Ghislain; Kay, Steve A; Pruneda-Paz, José L (2016) Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome. Methods Mol Biol 1398:107-18
Huang, He; Alvarez, Sophie; Bindbeutel, Rebecca et al. (2016) Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry. Mol Cell Proteomics 15:201-17
Nagel, Dawn H; Doherty, Colleen J; Pruneda-Paz, Jose L et al. (2015) Genome-wide identification of CCA1 targets uncovers an expanded clock network in Arabidopsis. Proc Natl Acad Sci U S A 112:E4802-10

Showing the most recent 10 out of 36 publications