Abstract

The goal of this application is to understand the roles in vertebrate development of the nodal and nodal-related genes, genes that encode secreted intercellular signaling proteins in the TGF-beta/BMP family. It is proposed to test the following hypotheses: (1) that Xnr1 and Xnr2 are essential for normal mesoderm induction and patterning in vivo; (2) that sinistral Xnr1 expression is a causative factor in the development of left-right morphological laterality in vertebrates, and (3) that the left-specific expression of Xnr1 is under transcriptional control via cis-acting Xnr1 gene sequences.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM056238-02
Application #
2734835
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Project Start
1997-07-01
Project End
2001-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Payne, Riley; Hoff, Henry; Roskowski, Anne et al. (2017) MICU2 Restricts Spatial Crosstalk between InsP3R and MCU Channels by Regulating Threshold and Gain of MICU1-Mediated Inhibition and Activation of MCU. Cell Rep 21:3141-3154
Halstead, Angela M; Wright, Christopher V E (2015) Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning. Mech Dev 136:155-65
Blum, Martin; Schweickert, Axel; Vick, Philipp et al. (2014) Symmetry breakage in the vertebrate embryo: when does it happen and how does it work? Dev Biol 393:109-23
Marjoram, Lindsay; Wright, Christopher (2011) Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus. Development 138:475-85
Westmoreland, Joby J; Takahashi, Shuji; Wright, Christopher V E (2007) Xenopus Lefty requires proprotein cleavage but not N-linked glycosylation to inhibit nodal signaling. Dev Dyn 236:2050-61
Ohi, Yuki; Wright, Christopher V E (2007) Anteriorward shifting of asymmetric Xnr1 expression and contralateral communication in left-right specification in Xenopus. Dev Biol 301:447-63
Cha, Young Ryun; Takahashi, Shuji; Wright, Christopher V E (2006) Cooperative non-cell and cell autonomous regulation of Nodal gene expression and signaling by Lefty/Antivin and Brachyury in Xenopus. Dev Biol 290:246-64
Ripley, Anna N; Osler, Megan E; Wright, Christopher V E et al. (2006) Xbves is a regulator of epithelial movement during early Xenopus laevis development. Proc Natl Acad Sci U S A 103:614-9
Erter, C E; Wilm, T P; Basler, N et al. (2001) Wnt8 is required in lateral mesendodermal precursors for neural posteriorization in vivo. Development 128:3571-83
Osada, S I; Saijoh, Y; Frisch, A et al. (2000) Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1. Development 127:2503-14

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