The long-term goal of this research is to understand how centrosomes in animal cells nucleate microtubules and how this nucleating activity is regulated by cell-cycle and developmental processes. The protein complex to be used in this study is the recently identified gamma-tubulin ring complex (gamma-TuRC) which can nucleate and bind to the minus ends of tits nucleated microtubules in vitro. Based on a number of lines of evidence it is hypothesized that the gamma-TuRC is the major microtubule nucleator at the centrosomes. The gamma-TuRC consists of gamma tubulin and at least five currently unidentified proteins (p183, p151, p98, p82 and p75s). The four aims of this proposal are to: 1) clone and biochemically characterize the currently unknown proteins in the gamma-TuRC; 2) elucidate the structural organization of gamma-TuRC; 3)reconstitute functional gamma TuRC in vitro; and 4)determine the detailed functions of the gamma-TuRC.
| Huang, Yuejia; Li, Teng; Ems-McClung, Stephanie C et al. (2018) Aurora A activation in mitosis promoted by BuGZ. J Cell Biol 217:107-116 |
| Zheng, Xiaobin; Hu, Jiabiao; Yue, Sibiao et al. (2018) Lamins Organize the Global Three-Dimensional Genome from the Nuclear Periphery. Mol Cell 71:802-815.e7 |
| Gigante, Crystal M; Dibattista, Michele; Dong, Frederick N et al. (2017) Lamin B1 is required for mature neuron-specific gene expression during olfactory sensory neuron differentiation. Nat Commun 8:15098 |
| Chen, Haiyang; Zheng, Xiaobin; Xiao, Danqing et al. (2016) Age-associated de-repression of retrotransposons in the Drosophila fat body, its potential cause and consequence. Aging Cell 15:542-52 |
| Tran, Joseph R; Zheng, Xiaobin; Zheng, Yixian (2016) Lamin-B1 contributes to the proper timing of epicardial cell migration and function during embryonic heart development. Mol Biol Cell 27:3956-3963 |
| Tran, Joseph R; Chen, Haiyang; Zheng, Xiaobin et al. (2016) Lamin in inflammation and aging. Curr Opin Cell Biol 40:124-130 |
| Zheng, Xiaobin; Yue, Sibiao; Chen, Haiyang et al. (2015) Low-Cell-Number Epigenome Profiling Aids the Study of Lens Aging and Hematopoiesis. Cell Rep 13:1505-1518 |
| Guo, Yuxuan; Zheng, Yixian (2015) Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell 26:3379-89 |
| Zheng, Xiaobin; Kim, Youngjo; Zheng, Yixian (2015) Identification of lamin B-regulated chromatin regions based on chromatin landscapes. Mol Biol Cell 26:2685-97 |
| Oakley, Berl R; Paolillo, Vitoria; Zheng, Yixian (2015) ?-Tubulin complexes in microtubule nucleation and beyond. Mol Biol Cell 26:2957-62 |
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