Abstract

In this application, the P.I. proposes a series of aims designed to address the localization of ASH1 mRNA in yeast cells. Ash1p is an asymmetrically localized protein, important in repressing mating type switching in the daughter cell. The asymmetry of this nuclear protein results from the localization of its mRNA at the distal cortex of a daughter cell, the bud, and the greater accumulation of this negative regulator of gene expression in daughter nuclei. In the first aim, cis-acting sequences and trans-acting factors will be isolated in order to determine the protein components of the mechanism.
Specific aim 2 proposes experiments to follow the transport, export and localization of this RNA from the site of its transcription to its final location at the cortex of the bud. In the final specific aim, the mechanism of nuclear export will be assessed to determine points in the pathway where rate-limitations may exist.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM057071-02
Application #
2857362
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1998-01-01
Project End
2001-12-31
Budget Start
1999-01-01
Budget End
1999-12-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Tutucci, Evelina; Vera, Maria; Biswas, Jeetayu et al. (2018) An improved MS2 system for accurate reporting of the mRNA life cycle. Nat Methods 15:81-89
Zhang, Qianjun; Meng, Xiuhua; Li, Delin et al. (2017) Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region of ASH1 mRNA represses localized translation of ASH1 in yeast cells. J Biol Chem 292:9787-9800
Wang, Guangli; Zeng, Yao; Chen, Shaoying et al. (2017) Localization of TFPI-2 in the nucleus modulates MMP-2 gene expression in breast cancer cells. Sci Rep 7:13575
Brickner, Donna Garvey; Sood, Varun; Tutucci, Evelina et al. (2016) Subnuclear positioning and interchromosomal clustering of the GAL1-10 locus are controlled by separable, interdependent mechanisms. Mol Biol Cell 27:2980-93
Vera, Maria; Biswas, Jeetayu; Senecal, Adrien et al. (2016) Single-Cell and Single-Molecule Analysis of Gene Expression Regulation. Annu Rev Genet 50:267-291
Smith, Carlas S; Preibisch, Stephan; Joseph, Aviva et al. (2015) Nuclear accessibility of ?-actin mRNA is measured by 3D single-molecule real-time tracking. J Cell Biol 209:609-19
Smith, Carlas; Lari, Azra; Derrer, Carina Patrizia et al. (2015) In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p. J Cell Biol 211:1121-30
Grimm, Jonathan B; English, Brian P; Chen, Jiji et al. (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods 12:244-50, 3 p following 250
Hocine, Sami; Vera, Maria; Zenklusen, Daniel et al. (2015) Promoter-Autonomous Functioning in a Controlled Environment using Single Molecule FISH. Sci Rep 5:9934
Buxbaum, Adina R; Haimovich, Gal; Singer, Robert H (2015) In the right place at the right time: visualizing and understanding mRNA localization. Nat Rev Mol Cell Biol 16:95-109

Showing the most recent 10 out of 54 publications