Posttranslational modification is a rapid and efficient way in which cells adapt to changes in their environment. Biologists at large are interested in advancing our understanding of the mechanisms through which chemical modifications exert their effects, and want to learn more about the impact that these modifications have on the dynamics of the complex metabolic network of the cell. The long-term goal of the work supported by GM62203 is to understand the contributions of the sirtuin- dependent protein acylation/deacylation system (SDPADS) to prokaryotic cell physiology. Previous NIH funding of this project allowed us to advance our understanding of the role of the SDPADS in Gram- negative and Gram-positive bacteria. We are now in an excellent position to perform structure-function analyses of the acetyltransferase of the SDPADS, to dissect the regulation of expression of the genes encoding SDPADS functions, to identify the function of previously unknown genes also involved in acetylation control of protein activity, to establish a role for N-Lys protein acetylation/deacetylation in the control of central metabolic pathways, and to determine if prokaryotes use the SDPADS to directly control gene expression by modulating the activity of transcription regulators. We will take a multidisciplinary approach to answer fundamental questions of prokaryotic cell physiology. The first two aims of this proposal focus on newly discovered effects of acetylation on metabolism and gene expression, and the last two aims focus on the biochemical and structural characterization of the SDPADS enzymes and on the regulation of expression of the genes encoding SDPADS functions. Given the ubiquity of the SDPADS, advances in this research area will have broad impact on our understanding of the physiology of all cells.

Public Health Relevance

Rapid control of biological activities of proteins is critical for the survival of cells in a changing environment. Posttranslational modifications of lysine residues have a profound effect on gene expression and metabolism. The proposed work will advance our understanding of the mechanism of function of the modifying/demodifying enzymes that comprise a system conserved in all forms of life. Regulation of expression of the genes encoding the modifying/demodifying enzymes is part of the proposed studies. In this project we seek to validate putative new protein substrates of the system, including DNA-binding regulatory proteins.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Special Emphasis Panel (ZRG1-IDM-S (02))
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Hagan, Ann A
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University of Wisconsin Madison
Schools of Earth Sciences/Natur
United States
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VanDrisse, Chelsey M; Escalante-Semerena, Jorge C (2018) In Streptomyces lividans, acetyl-CoA synthetase activity is controlled by O-serine and N? -lysine acetylation. Mol Microbiol 107:577-594
VanDrisse, Chelsey M; Escalante-Semerena, Jorge C (2018) Small-Molecule Acetylation Controls the Degradation of Benzoate and Photosynthesis in Rhodopseudomonas palustris. MBio 9:
Burckhardt, Rachel M; Escalante-Semerena, Jorge C (2017) In Bacillus subtilis, the SatA (Formerly YyaR) Acetyltransferase Detoxifies Streptothricin via Lysine Acetylation. Appl Environ Microbiol 83:
VanDrisse, Chelsey M; Parks, Anastacia R; Escalante-Semerena, Jorge C (2017) A Toxin Involved in Salmonella Persistence Regulates Its Activity by Acetylating Its Cognate Antitoxin, a Modification Reversed by CobB Sirtuin Deacetylase. MBio 8:
Rocco, Christopher J; Wetterhorn, Karl M; Garvey, Graeme S et al. (2017) The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. PLoS One 12:e0188130
VanDrisse, C M; Escalante-Semerena, J C (2016) New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica. Plasmid 86:1-6
VanDrisse, Chelsey M; Hentchel, Kristy L; Escalante-Semerena, Jorge C (2016) Phosphinothricin Acetyltransferases Identified Using In Vivo, In Vitro, and Bioinformatic Analyses. Appl Environ Microbiol 82:7041-7051
Hentchel, Kristy L; Thao, Sandy; Intile, Peter J et al. (2015) Deciphering the Regulatory Circuitry That Controls Reversible Lysine Acetylation in Salmonella enterica. MBio 6:e00891
Stuecker, Tara N; Bramhacharya, Shanti; Hodge-Hanson, Kelsey M et al. (2015) Phylogenetic and amino acid conservation analyses of bacterial L-aspartate-?-decarboxylase and of its zymogen-maturation protein reveal a putative interaction domain. BMC Res Notes 8:354
Hentchel, Kristy L; Escalante-Semerena, Jorge C (2015) In Salmonella enterica, the Gcn5-related acetyltransferase MddA (formerly YncA) acetylates methionine sulfoximine and methionine sulfone, blocking their toxic effects. J Bacteriol 197:314-25

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