The long-term goal of this research is to explore the molecular mechanisms that bacteria use for intra-and inter-species cell-cell communication. The objective of this proposal is to identify and study the model Vibrio harveyi signal transduction pathway that links detection of extracellular quorum sensing signal molecules termed Al-I and Al-2 to target gene responses. Definition of the genes, proteins, protein-protein interactions, protein modifications and signaling molecules involved in this cell-cell communication system will lead to a molecular understanding of how signals are detected and how this information is integrated, processed, and transduced to control behavior on a community scale. Analyzing the V. harveyi quorum sensing signaling circuit should be valuable for understanding how bacteria perceive and react to their environment, how they respond to temporally coincident cues, and how they communicate with one another to coordinate behavior. Since the regulatory process is complex and involves both intra-species, inter-species, intra-cellular and inter-cellular signal transmission, interesting new mechanisms should be revealed

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM065859-03
Application #
6783371
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Anderson, James J
Project Start
2002-08-01
Project End
2006-07-31
Budget Start
2004-08-01
Budget End
2005-07-31
Support Year
3
Fiscal Year
2004
Total Cost
$408,576
Indirect Cost
Name
Princeton University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
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Nadell, Carey D; Ricaurte, Deirdre; Yan, Jing et al. (2017) Flow environment and matrix structure interact to determine spatial competition in Pseudomonas aeruginosa biofilms. Elife 6:
Høyland-Kroghsbo, Nina M; Paczkowski, Jon; Mukherjee, Sampriti et al. (2017) Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system. Proc Natl Acad Sci U S A 114:131-135
Hurley, Amanda; Bassler, Bonnie L (2017) Asymmetric regulation of quorum-sensing receptors drives autoinducer-specific gene expression programs in Vibrio cholerae. PLoS Genet 13:e1006826
Yan, Jing; Sharo, Andrew G; Stone, Howard A et al. (2016) Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging. Proc Natl Acad Sci U S A 113:E5337-43

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