Some insects compete with us for food and transmit human and livestock diseases, but other insects are beneficial. Therefore, there has been a continuous demand for the development of insect control methods that are target-specific. Juvenile hormone (JH) and ecdysteroids are the major hormones that regulate molting, metamorphosis and reproduction in insects. Since these hormones are not present in vertebrates, they represent attractive targets for the development of insect control methods. Hindering this effort is the lack of understanding of the molecular basis of JH action. The main objective of this proposal is to identify genes that play critical roles in JH action. JH response elements (JHRE) identified in the promoter region of JH- and 20-hydroxyecdysone (20E)-responsive JH esterase (jhe) gene and ecdysone receptor (EcR) deficient Drosophila melanogaster cell line (L57) were used to develop a robust system that can be used for studying the molecular basis of JH action. In L57 cells, a reporter gene placed under the control of JHRE is induced by JH, and the JH induction was suppressed by 20E. The nuclear proteins isolated from L57 cells bind to JHRE and 20E in the presence of EcR can prevent this binding. In L57 cells, EcR and DSP do not bind directly to JHRE and are not directly involved in JH induction of a reporter gene regulated by JHRE. Identification of proteins that bind to JHRE is critical for understanding JH action. The two specific aims of the proposal are: (1) to identify genes that play key roles in JH action; and (2) to determine the role of identified genes in JH action. The genes that are involved in JH induction of JHRE regulated reporter gene will be identified using RNAi and yeast assays. The identified gene will be characterized by studying the binding of expressed proteins to JHRE and labeled JH, expression of their mRNA and proteins during D. melanogaster development, and analysis of D. melanogaster mutants deficient in identified genes. JH analogs are being used for mosquito control, but some mosquitoes are already developing resistance to these analogs. The results from these studies will aid in understanding the mode of action of JH analogs, thus facilitating their judicious use. The genes identified will be useful to develop screening assays to identify new insect disease vector control agents. The receptors can also be used in medicine by developing gene switches for various applications such as gene therapy, drug discovery and therapeutic protein production.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM070559-04
Application #
7340781
Study Section
Special Emphasis Panel (ZRG1-TMP (99))
Program Officer
Chin, Jean
Project Start
2005-02-01
Project End
2010-01-31
Budget Start
2008-02-01
Budget End
2009-01-31
Support Year
4
Fiscal Year
2008
Total Cost
$193,429
Indirect Cost
Name
University of Kentucky
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506
Xu, Jingjing; Roy, Amit; Palli, Subba Reddy (2018) CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum. Sci Rep 8:1426
Singh, Satnam; Gupta, Mridula; Pandher, Suneet et al. (2018) Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida). PLoS One 13:e0191116
Yoon, June-Sun; Sahoo, Dipak K; Maiti, Indu B et al. (2018) Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite. Sci Rep 8:14687
Yoon, June-Sun; Mogilicherla, Kanakachari; Gurusamy, Dhandapani et al. (2018) Double-stranded RNA binding protein, Staufen, is required for the initiation of RNAi in coleopteran insects. Proc Natl Acad Sci U S A 115:8334-8339
Niu, Kangkang; Zhang, Xiaojuan; Deng, Huimin et al. (2018) BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori. Nucleic Acids Res 46:1710-1723
Schoville, Sean D; Chen, Yolanda H; Andersson, Martin N et al. (2018) A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Sci Rep 8:1931
Liu, Suning; Li, Kang; Gao, Yue et al. (2018) Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila. Proc Natl Acad Sci U S A 115:139-144
Palli, Subba Reddy (2017) New roles for old actors, ROS and PRMT1. Proc Natl Acad Sci U S A 114:10810-10812
Roy, Amit; George, Smitha; Palli, Subba Reddy (2017) Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. BMC Genomics 18:996
Zeng, Baosheng; Huang, Yuping; Xu, Jun et al. (2017) The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm, Bombyx mori. J Biol Chem 292:11659-11669

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