We established that a multiprotein chaperone complex containing Hsp90/Hsp70 chaperones plays an essential role in maintaining protein quality control of neuronal NO synthase (nNOS) and other P450 cytochromes. In the last grant cycle, we turned our focus to determine the structure of nNOS by single particle EM and cryo-EM methods to help elucidate what triggers chaperone recognition of nNOS. In the course of these studies, we serendipitously discovered that full-length CYP2B4:cytochrome P450 reductase (CPR) complexes could form in amphipols, which are surfactants that self-assemble and stabilize membrane proteins in the absence of detergent. Remarkably, these complexes were fully functional, stable, and visible as tetramers by single particle EM methods, and found to contain equimolar amounts of P450 and CPR. Thus, in the current proposal, we aim to determine the first structure of a microsomal P450 in complex with CPR. Our prior success in elucidating the first full-length dimeric structure of nNOS and P450 BM3 by these single particle methods provides confidence in this undertaking. Currently we have resolved the structure of the oxygenase domain of full-length nNOS to ?5 resolution by cryo-EM methods. It is noteworthy that a tetrameric complex of P450:CPR complex is analogous to the dimeric architecture of nNOS and BM3, both having a P450 and CPR domain in each monomer. We have a collaborative team of EM experts whose knowledge will synergize with our expertise in P450 and multi-protein complexes to tackle this exciting but challenging project. Also in prior grant cycles, we showed that Hsp90 is needed for cellular heme insertion into heme-deficient apo-nNOS and more recently the Stuehr lab has shown that Hsp90 is needed to insert heme into guanylate cyclase, hemoglobin, and inducible NO synthase. Thus, premise exists for a chaperone complex that is a ?heme insertase?. Recently, we discovered that the cellular proteins that immunoprecipitate with apo-nNOS are stably bound and catalyze heme insertion into apo-nNOS. Hsp90 and Hsp70 are bound to apo-nNOS and play a key role in heme insertion but Hsp90 and Hsp70 chaperones alone are not sufficient. In the current proposal, we seek to identify the other proteins that make up the heme insertase activity, in part by identification of the co-immunoprecipitated proteins by LC-MS/MS. Moreover, we will define how the chaperone-based heme insertase complex is assembled. The successful completion of the aims will provide a groundbreaking new platform for the study of microsomal P450s, elucidate the first structure of a P450:CPR complex, and characterize the protein machinery that inserts heme into hemeproteins.
This research furthers our understanding of the fundamental process of how proteins, such as Hsp90 and Hsp70 chaperones, work together to assemble hemeproteins, such as NO synthase, and promote their proper function. We aim to use single particle imaging techniques to define the architecture of these multi-protein complexes, including for the first time a liver microsomal P450: NADPH P450 reductase complex. These studies will lead to a better understanding of how important hemeproteins, such as NO synthase and liver drug metabolizing P450 enzymes, are regulated by their partner proteins.
|Zhang, Haoming; Yokom, Adam L; Cheng, Shen et al. (2018) The full-length cytochrome P450 enzyme CYP102A1 dimerizes at its reductase domains and has flexible heme domains for efficient catalysis. J Biol Chem 293:7727-7736|
|Morishima, Yoshihiro; Mehta, Ranjit K; Yoshimura, Miyako et al. (2018) Chaperone Activity and Dimerization Properties of Hsp90? and Hsp90? in Glucocorticoid Receptor Activation by the Multiprotein Hsp90/Hsp70-Dependent Chaperone Machinery. Mol Pharmacol 94:984-991|
|Capper, C P; Liu, J; McIntosh, L R et al. (2018) Functional characterization of the G162R and D216H genetic variants of human CYP17A1. J Steroid Biochem Mol Biol 178:159-166|
|Gates, Stephanie N; Yokom, Adam L; Lin, JiaBei et al. (2017) Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104. Science 357:273-279|
|Morishima, Yoshihiro; Zhang, Haoming; Lau, Miranda et al. (2016) Improved method for assembly of hemeprotein neuronal NO-synthase heterodimers. Anal Biochem 511:24-6|
|Yokom, Adam L; Gates, Stephanie N; Jackrel, Meredith E et al. (2016) Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation. Nat Struct Mol Biol 23:830-7|
|Zhang, Haoming; Lauver, D Adam; Wang, Hui et al. (2016) Significant Improvement of Antithrombotic Responses to Clopidogrel by Use of a Novel Conjugate as Revealed in an Arterial Model of Thrombosis. J Pharmacol Exp Ther 359:11-7|
|Pratt, William B; Gestwicki, Jason E; Osawa, Yoichi et al. (2015) Targeting Hsp90/Hsp70-based protein quality control for treatment of adult onset neurodegenerative diseases. Annu Rev Pharmacol Toxicol 55:353-71|
|Lee, Sung Ki; Tatiyaborworntham, Nantawat; Grunwald, Eric W et al. (2015) Myoglobin and haemoglobin-mediated lipid oxidation in washed muscle: observations on crosslinking, ferryl formation, porphyrin degradation, and haemin loss rate. Food Chem 167:258-63|
|Pratt, William B; Morishima, Yoshihiro; Gestwicki, Jason E et al. (2014) A model in which heat shock protein 90 targets protein-folding clefts: rationale for a new approach to neuroprotective treatment of protein folding diseases. Exp Biol Med (Maywood) 239:1405-13|
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