Our aim is to advance genome analysis by providing interactive exploratory visualization tools enabling researchers to refine, correct and augment initial automated results. The cost of sequencing a genome has been dramatically reduced by several orders of magnitude in the last decade, and the natural consequence is that more and more researchers are sequencing more and more new genomes, both within populations and across every species. Because computational genome analysis remains an imperfect art each new exome or genome that is sequenced requires visualization. With effective visualization, genome interpretation can take advantage of human perceptual capabilities to integrate information. Web Apollo will provide an easy to use, web-based environment offering multiple, distributed users high-performance visualization for interactive exploration of genome data, and the tools needed for producing highly accurate annotations that are maximally informative for downstream analysis. This project will enable every member of the burgeoning genomic community to fully mobilize molecular research data, whether collected by individual researchers or made available from public aggregation sites to benefit investigations into genetics and human diseases. This project will enable researchers to (i) Visually project variants onto protein displays to see intersection with protein features such as active sites and secondary structure to assess biological impact; (ii) Improve exonintron accuracy for protein coding transcripts via direct comparison to related proteins in an integrated multiple sequence alignment (MSA) view; (iii) Explore the genome using bookmarking of key sites for rapid navigation; (iv) Analyze the data interactively by folding of the genome to bring genes with shared attributes such as paralogy or shared phenotypes and the variants within them in the same visual field ; (vi) Collaborate securely in real-time with selected team members over the Internet using hybrid clouds for private data and freely-hosted servers for public data.
Apollo will provide a suite of collaborative and interactive investigation tools to understand the relationship between DNA variation and human disease. Its real- time collaborative environment mobilizes more researchers, who can easily connect with one another and working together as a concerted team more rapidly gain insight into the growing volume of genomic information. Apollo's visual exploratory tools gives maximum leverage to human cognitive abilities to pinpoint unique differences and recognize correlations across the genome in their investigations into the genetic orig ins of complex diseases.
