This administrative supplement request is in response to NOT-GM-19-013, Notice of Availability of Administrative Supplements for Equipment Purchases for NIGMS Awardees, for the purchase of an AKTA pure 25M FPLC system. These administrative supplement funds will allow the purchase of an instrument essential for the purification of the telomerase enzyme and recombinant proteins. This new instrument will replace a nonfunctional instrument in the sponsored laboratory at Arizona State University and expedite the progress of the proposed research projects.
Telomerase is a specialized RNA-dependent DNA polymerase that adds DNA repeats to chromosome ends and viewed as an important drug target for anti-ageing and anti-cancer therapies. This administrative supplement funds will allow the purchase of an instrument essential for the purification of the telomerase enzyme and recombinant proteins. This new instrument will replace a nonfunctional instrument in the sponsored laboratory at Arizona State University and expedite the progress of the proposed research projects.
| Chen, Yinnan; Podlevsky, Joshua D; Logeswaran, Dhenugen et al. (2018) A single nucleotide incorporation step limits human telomerase repeat addition activity. EMBO J 37: |
| Huang, Jing; Bley, Christopher J; Rand, Dustin P et al. (2017) In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits. Methods Mol Biol 1587:161-169 |
| Donaires, Flávia S; Scatena, Natália F; Alves-Paiva, Raquel M et al. (2017) Telomere biology and telomerase mutations in cirrhotic patients with hepatocellular carcinoma. PLoS One 12:e0183287 |
| Podlevsky, Joshua D; Li, Yang; Chen, Julian J-L (2016) Structure and function of echinoderm telomerase RNA. RNA 22:204-15 |
| Podlevsky, Joshua D; Chen, Julian J-L (2016) Evolutionary perspectives of telomerase RNA structure and function. RNA Biol 13:720-32 |
| Podlevsky, Joshua D; Li, Yang; Chen, Julian J-L (2016) The functional requirement of two structural domains within telomerase RNA emerged early in eukaryotes. Nucleic Acids Res 44:9891-9901 |
| Qi, Xiaodong; Rand, Dustin P; Podlevsky, Joshua D et al. (2015) Prevalent and distinct spliceosomal 3'-end processing mechanisms for fungal telomerase RNA. Nat Commun 6:6105 |
| Huang, Jing; Bley, Christopher J; Rand, Dustin P et al. (2015) Sample Preparation of Telomerase Subunits for Crystallization. Bio Protoc 5: |
| Stanley, Susan E; Chen, Julian J L; Podlevsky, Joshua D et al. (2015) Telomerase mutations in smokers with severe emphysema. J Clin Invest 125:563-70 |
| Huang, Jing; Brown, Andrew F; Wu, Jian et al. (2014) Structural basis for protein-RNA recognition in telomerase. Nat Struct Mol Biol 21:507-12 |
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