Abstract

Adhesion among chick embryo nerve cells requires the function of a cell surface polypeptide called CAM. Specific antibodies against CAM inhibit neural cell aggregation, the fasciculation of neurites, sorting out of retinal cells in aggregates, and also perturb the formation of cell and neurite layers in cultured retinal tissue. CAM-mediated adhesion has also been shown to influence the NGF-induced growth, attraction and retraction of nerve fibers. Recent studies indicate that adhesion among embryonic liver cells occurs by a different mechanism involving another cell surface protein that is antigenically distinct from CAM. During the next year our research will include a detailed chemical characterization of CAM, including structural comparisons to the liver cell adhesion molecule, as well as experiments on the precise function of CAM in the mechanism of cell-cell binding. These studies should contribute to a fundamental understanding of how cell-cell interactions affect tissue formation during embryogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD009635-11
Application #
3311133
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1978-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Tsatmali, Marina; Walcott, Elisabeth C; Makarenkova, Helen et al. (2006) Reactive oxygen species modulate the differentiation of neurons in clonal cortical cultures. Mol Cell Neurosci 33:345-57
Aschrafi, Armaz; Cunningham, Bruce A; Edelman, Gerald M et al. (2005) The fragile X mental retardation protein and group I metabotropic glutamate receptors regulate levels of mRNA granules in brain. Proc Natl Acad Sci U S A 102:2180-5
Vanderklish, P W; Edelman, G M (2005) Differential translation and fragile X syndrome. Genes Brain Behav 4:360-84
Atkins, Annette R; Gallin, Warren J; Owens, Geoffrey C et al. (2004) Neural cell adhesion molecule (N-CAM) homophilic binding mediated by the two N-terminal Ig domains is influenced by intramolecular domain-domain interactions. J Biol Chem 279:49633-43
Chappell, Stephen A; Edelman, Gerald M; Mauro, Vincent P (2004) Biochemical and functional analysis of a 9-nt RNA sequence that affects translation efficiency in eukaryotic cells. Proc Natl Acad Sci U S A 101:9590-4
Rogers Jr, George W; Edelman, Gerald M; Mauro, Vincent P (2004) Differential utilization of upstream AUGs in the beta-secretase mRNA suggests that a shunting mechanism regulates translation. Proc Natl Acad Sci U S A 101:2794-9
Smart, Fiona M; Edelman, Gerald M; Vanderklish, Peter W (2003) BDNF induces translocation of initiation factor 4E to mRNA granules: evidence for a role of synaptic microfilaments and integrins. Proc Natl Acad Sci U S A 100:14403-8
Mistry, Sanjay K; Keefer, Edward W; Cunningham, Bruce A et al. (2002) Cultured rat hippocampal neural progenitors generate spontaneously active neural networks. Proc Natl Acad Sci U S A 99:1621-6
Owens, G C; Mistry, S; Edelman, G M et al. (2002) Efficient marking of neural stem cell-derived neurons with a modified murine embryonic stem cell virus, MESV2. Gene Ther 9:1044-8
Atkins, A R; Chung, J; Deechongkit, S et al. (2001) Solution structure of the third immunoglobulin domain of the neural cell adhesion molecule N-CAM: can solution studies define the mechanism of homophilic binding? J Mol Biol 311:161-72

Showing the most recent 10 out of 102 publications