This project will use stable isotope tracers and combined gas chromatography-mass spectrometry to 1) study the physiology of amino acid transport and disposal, the conversion of essential to non-essential amino acds, and the fate of non-essential amino acid nitrogen in man; 2) to evaluate the effects of nutrition, energy balance, and aging on these rates; and 3) to investigate the pathology of these processes in diabetes and uremia, both disorders where protein synthesis is impaired and degradation accelerated. In the former entity, however, amino acid nitrogen is wasted in the urine while in the latter group, renal failure removes the effective route of nitrogen disposal. These results will be compared with correlates obtained under similar pathophysiological perturbations in the isolated rat epitrochlaris muscle. Taken together, these data should allow a more integrated picture of the quantitative dynamics of individual amino acid metabolism in man.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
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Metabolism Study Section (MET)
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Washington University
Schools of Medicine
Saint Louis
United States
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Staten, M A; Matthews, D E; Cryer, P E et al. (1989) Epinephrine's effect on metabolic rate is independent of changes in plasma insulin or glucagon. Am J Physiol 257:E185-92
Fukagawa, N K; Minaker, K L; Rowe, J W et al. (1988) Glucose and amino acid metabolism in aging man: differential effects of insulin. Metabolism 37:371-7
Darmaun, D; Matthews, D E; Desjeux, J F et al. (1988) Glutamine and glutamate nitrogen exchangeable pools in cultured fibroblasts: a stable isotope study. J Cell Physiol 134:143-8
Darmaun, D; Matthews, D E; Bier, D M (1988) Physiological hypercortisolemia increases proteolysis, glutamine, and alanine production. Am J Physiol 255:E366-73
Fukagawa, N K; Minaker, K L; Rowe, J W et al. (1987) Plasma tryptophan and total neutral amino acid levels in men: influence of hyperinsulinemia and age. Metabolism 36:683-6
Bougneres, P F; Balasse, E O; Ferre, P et al. (1986) Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer. J Lipid Res 27:215-20
Meguid, M M; Matthews, D E; Bier, D M et al. (1986) Leucine kinetics at graded leucine intakes in young men. Am J Clin Nutr 43:770-80
Meredith, C N; Wen, Z M; Bier, D M et al. (1986) Lysine kinetics at graded lysine intakes in young men. Am J Clin Nutr 43:787-94
Meguid, M M; Matthews, D E; Bier, D M et al. (1986) Valine kinetics at graded valine intakes in young men. Am J Clin Nutr 43:781-6
Bougneres, P F; Lemmel, C; Ferre, P et al. (1986) Ketone body transport in the human neonate and infant. J Clin Invest 77:42-8

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