Abstract

This project will use stable isotope tracers and combined gas chromatography-mass spectrometry to 1) study the physiology of amino acid transport and disposal, the conversion of essential to non-essential amino acds, and the fate of non-essential amino acid nitrogen in man; 2) to evaluate the effects of nutrition, energy balance, and aging on these rates; and 3) to investigate the pathology of these processes in diabetes and uremia, both disorders where protein synthesis is impaired and degradation accelerated. In the former entity, however, amino acid nitrogen is wasted in the urine while in the latter group, renal failure removes the effective route of nitrogen disposal. These results will be compared with correlates obtained under similar pathophysiological perturbations in the isolated rat epitrochlaris muscle. Taken together, these data should allow a more integrated picture of the quantitative dynamics of individual amino acid metabolism in man.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD010667-09
Application #
3311351
Study Section
Metabolism Study Section (MET)
Project Start
1978-02-01
Project End
1986-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Staten, M A; Matthews, D E; Cryer, P E et al. (1989) Epinephrine's effect on metabolic rate is independent of changes in plasma insulin or glucagon. Am J Physiol 257:E185-92
Darmaun, D; Matthews, D E; Desjeux, J F et al. (1988) Glutamine and glutamate nitrogen exchangeable pools in cultured fibroblasts: a stable isotope study. J Cell Physiol 134:143-8
Darmaun, D; Matthews, D E; Bier, D M (1988) Physiological hypercortisolemia increases proteolysis, glutamine, and alanine production. Am J Physiol 255:E366-73
Fukagawa, N K; Minaker, K L; Rowe, J W et al. (1988) Glucose and amino acid metabolism in aging man: differential effects of insulin. Metabolism 37:371-7
Fukagawa, N K; Minaker, K L; Rowe, J W et al. (1987) Plasma tryptophan and total neutral amino acid levels in men: influence of hyperinsulinemia and age. Metabolism 36:683-6
Darmaun, D; Matthews, D E; Bier, D M (1986) Glutamine and glutamate kinetics in humans. Am J Physiol 251:E117-26
Yang, R D; Matthews, D E; Bier, D M et al. (1986) Response of alanine metabolism in humans to manipulation of dietary protein and energy intakes. Am J Physiol 250:E39-46
Bougneres, P F; Balasse, E O; Ferre, P et al. (1986) Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer. J Lipid Res 27:215-20
Meguid, M M; Matthews, D E; Bier, D M et al. (1986) Leucine kinetics at graded leucine intakes in young men. Am J Clin Nutr 43:770-80
Meredith, C N; Wen, Z M; Bier, D M et al. (1986) Lysine kinetics at graded lysine intakes in young men. Am J Clin Nutr 43:787-94

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