Abstract

The objective of the proposed research is to delineate the GnRH self-priming pathway and its intersection with progesterone receptor PR.
The specific aims are: 10 to determine steady state levels of PR mRNA and levels of PR-a and PR-b isoforms and their regulation by estrogen in gonadotropes and a newly developed cell line, LBT2 cells; 2) to test whether the temporal pattern of PKA and PKC activities interact to influence PR mediated self-priming; 3) to test the hypothesis that PKA is a mediator of self priming, using a single cell model in which membrane capacitance and intracellular Ca are measured as indices of exocytosis and secretion; and 4) to use microinjection of PR antibodies or antisense oligonucleotides into single cells to test the hypothesis that PKA and PKC interact at the level of PR to induce self priming.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD012137-16
Application #
2609053
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1979-12-01
Project End
2000-11-30
Budget Start
1997-12-01
Budget End
1998-11-30
Support Year
16
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Physiology
Type
Schools of Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Yuen, Tony; Choi, Soon Gang; Pincas, Hanna et al. (2012) Optimized amplification and single-cell analysis identify GnRH-mediated activation of Rap1b in primary rat gonadotropes. Mol Cell Endocrinol 350:10-9
Waring, Dennis W; Turgeon, Judith L (2009) Ca2+-activated K+ channels in gonadotropin-releasing hormone-stimulated mouse gonadotrophs. Endocrinology 150:2264-72
Turgeon, Judith L; Carr, Molly C; Maki, Pauline M et al. (2006) Complex actions of sex steroids in adipose tissue, the cardiovascular system, and brain: Insights from basic science and clinical studies. Endocr Rev 27:575-605
Turgeon, Judith L; Waring, Dennis W (2006) Differential expression and regulation of progesterone receptor isoforms in rat and mouse pituitary cells and LbetaT2 gonadotropes. J Endocrinol 190:837-46
Waring, Dennis W; Turgeon, Judith L (2006) Estradiol inhibition of voltage-activated and gonadotropin-releasing hormone-induced currents in mouse gonadotrophs. Endocrinology 147:5798-805
Turgeon, Judith L; McDonnell, Donald P; Martin, Kathryn A et al. (2004) Hormone therapy: physiological complexity belies therapeutic simplicity. Science 304:1269-73
Turgeon, J L; Shyamala, G; Waring, D W (2001) PR localization and anterior pituitary cell populations in vitro in ovariectomized wild-type and PR-knockout mice. Endocrinology 142:4479-85
Turgeon, J L; Waring, D W (2001) Luteinizing hormone secretion from wild-type and progesterone receptor knockout mouse anterior pituitary cells. Endocrinology 142:3108-15
Turgeon, J L; Waring, D W (2000) Progesterone regulation of the progesterone receptor in rat gonadotropes. Endocrinology 141:3422-9
Turgeon, J L; Van Patten, S M; Shyamala, G et al. (1999) Steroid regulation of progesterone receptor expression in cultured rat gonadotropes. Endocrinology 140:2318-25

Showing the most recent 10 out of 24 publications