Abstract

The sex steroid hormones (estrogen and progesterone) stimulate growth, maturation, and the development of new biochemical capacities in their reproductive organs. In chick oviduct tissue, estrogen and progesterone can stimulate the synthesis of egg white proteins. Substantial evidence has accumulated to support the notion that transcription is the primary site for steroid hormone regulation of gene expression. In order to understand the transcriptional control mechanisms in thi system, it is necessary to develop a cell-free system reconstituted from purified components so that an in vivo event can be duplicated. In this proposal we design experiments to isolate, purify, and study the mechanism of action of transcriptional factors for polymerase II genes. The studies will involve various aspects of biochemistry, molecular biology, and some immunochemistry. It is expected that the results derived from this project will be relevant to the understanding of action of steroid hormones on maternal physiology and fetal development. The following proposal should also be pertinent to development of more precise hypothesis for the mechanism of action of hormones in general, and more specifically, steroid hormones.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD017379-03
Application #
3314369
Study Section
Genetics Study Section (GEN)
Project Start
1983-02-01
Project End
1986-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Wu, San-Pin; Kao, Chung-Yang; Wang, Leiming et al. (2015) Increased COUP-TFII expression in adult hearts induces mitochondrial dysfunction resulting in heart failure. Nat Commun 6:8245
Tang, Ke; Rubenstein, John L R; Tsai, Sophia Y et al. (2012) COUP-TFII controls amygdala patterning by regulating neuropilin expression. Development 139:1630-9
Lin, Fu-Jung; You, Li-Ru; Yu, Cheng-Tai et al. (2012) Endocardial cushion morphogenesis and coronary vessel development require chicken ovalbumin upstream promoter-transcription factor II. Arterioscler Thromb Vasc Biol 32:e135-46
Yu, Cheng-Tai; Tang, Ke; Suh, Jae Mi et al. (2012) COUP-TFII is essential for metanephric mesenchyme formation and kidney precursor cell survival. Development 139:2330-9
Lin, Fu-Jung; Qin, Jun; Tang, Ke et al. (2011) Coup d'Etat: an orphan takes control. Endocr Rev 32:404-21
Xie, Xin; Qin, Jun; Lin, Sue-Hwa et al. (2011) Nuclear receptor chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) modulates mesenchymal cell commitment and differentiation. Proc Natl Acad Sci U S A 108:14843-8
Qin, Jun; Chen, Xinpu; Xie, Xin et al. (2010) COUP-TFII regulates tumor growth and metastasis by modulating tumor angiogenesis. Proc Natl Acad Sci U S A 107:3687-92
Lin, Fu-Jung; Chen, Xinpu; Qin, Jun et al. (2010) Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J Clin Invest 120:1694-707
Tang, Ke; Xie, Xin; Park, Joo-In et al. (2010) COUP-TFs regulate eye development by controlling factors essential for optic vesicle morphogenesis. Development 137:725-34
Lee, Dong-Kee; Kurihara, Isao; Jeong, Jae-Wook et al. (2010) Suppression of ERalpha activity by COUP-TFII is essential for successful implantation and decidualization. Mol Endocrinol 24:930-40

Showing the most recent 10 out of 54 publications