Abstract

In the proposed research, the function of sperm membrane autoantigens during fettilization will be determined using mouse fertilization, both in vitro and in vivo. A set of intrinsic sperm membrane autoantigens, selected on the basis of an effect upon sperm function during fertilization, will be defined with the aid of monoclonal antibodies produced by syngeneic immunization with mouse testis membranes. Furthermore, the behavior of the specific functional autoantigens during capacitation, zona binding, acrosome reaction induction, zona penetration and sperm-egg membrane fusion will be assessed by labeled monoclonal antibody probes. Finally, the lipid composition of the sperm plasma membrane will be altered to determine the role of sperm plasma membrane lipid during fertilization. The significance of this proposal lies in its examination of individual sperm membrane autoantigens with defined functions during specific, separable, steps of the fertilization process in the mouse.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018201-04
Application #
3315219
Study Section
Reproductive Biology Study Section (REB)
Project Start
1983-04-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Tomes, C N; Roggero, C M; De Blas, G et al. (2004) Requirement of protein tyrosine kinase and phosphatase activities for human sperm exocytosis. Dev Biol 265:399-415
Kiefer, Susan McLeskey; Saling, Patricia (2002) Proteolytic processing of human zona pellucida proteins. Biol Reprod 66:407-14
Tomes, C N; Carballada, R; Moses, D F et al. (1998) Treatment of human spermatozoa with seminal plasma inhibits protein tyrosine phosphorylation. Mol Hum Reprod 4:17-25
Carballada, R; Saling, P M (1997) Regulation of mouse epididymal epithelium in vitro by androgens, temperature and fibroblasts. J Reprod Fertil 110:171-81
Tomes, C N; McMaster, C R; Saling, P M (1996) Activation of mouse sperm phosphatidylinositol-4,5 bisphosphate-phospholipase C by zona pellucida is modulated by tyrosine phosphorylation. Mol Reprod Dev 43:196-204
Leyton, L; Tomes, C; Saling, P (1995) LL95 monoclonal antibody mimics functional effects of ZP3 on mouse sperm: evidence that the antigen recognized is not hexokinase. Mol Reprod Dev 42:347-58
Burks, D J; Carballada, R; Moore, H D et al. (1995) Interaction of a tyrosine kinase from human sperm with the zona pellucida at fertilization. Science 269:83-6
Beebe, S J; Leyton, L; Burks, D et al. (1992) Recombinant mouse ZP3 inhibits sperm binding and induces the acrosome reaction. Dev Biol 151:48-54
Wolf, D E; McKinnon, C A; Leyton, L et al. (1992) Protein dynamics in sperm membranes: implications for sperm function during gamete interaction. Mol Reprod Dev 33:228-34
Saling, P M (1991) How the egg regulates sperm function during gamete interaction: facts and fantasies. Biol Reprod 44:246-51

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