Abstract

The goal is to determine the structure of the LH/hCG receptor and the nature of its interactions with the hormone and associated molecules including adenylate cyclase. We will pursue the following specific objectives: 1. Micropurification and characterization of receptor subunits (peptide maps, finger-prints, amino acid sequence and ADP-ribosylation). 2. Determination of receptor subunit organization (cytoplasmic and extracellular domains, neighboring subunits, location of subunit crosslinks, phosporylation, fatty acylation and the presence of carbohydrates). 3. Study of hormone-receptor interactions; sites in the hormone crosslinked to the receptor, the preference and the order of the affinity labeling of receptor subunits by each of the hormone (LH, hCG and deglycosylated hCG) subunits. 4. Study of interactions of the receptor with the adenylate cyclase system. 5. Further preparation of monoclaonal antibodies to the receptor.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018702-13
Application #
3315830
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1977-01-12
Project End
1991-12-31
Budget Start
1989-01-01
Budget End
1989-12-31
Support Year
13
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Wyoming
Department
Type
Earth Sciences/Resources
DUNS #
069690956
City
Laramie
State
WY
Country
United States
Zip Code
82071

  Publications

Youn, HyeSook; Ji, Inhae; Ji, Hanlee P et al. (2007) Under-expression of Kalirin-7 Increases iNOS activity in cultured cells and correlates to elevated iNOS activity in Alzheimer's disease hippocampus. J Alzheimers Dis 12:271-81
Jeoung, MyoungKun; Lee, ChangWoo; Ji, Inhae et al. (2007) Trans-activation, cis-activation and signal selection of gonadotropin receptors. Mol Cell Endocrinol 260-262:137-43
Youn, HyeSook; Jeoung, MyoungKun; Koo, YongBum et al. (2007) Kalirin is under-expressed in Alzheimer's disease hippocampus. J Alzheimers Dis 11:385-97
Youn, HyeSook; Koo, YongBum; Ji, Inhae et al. (2005) An upstream initiator-like element suppresses transcription of the rat luteinizing hormone receptor gene. Mol Endocrinol 19:1318-28
Lee, ChangWoo; Ji, Inhae; Ji, Tae H (2004) Distinct mechanisms of cAMP induction by constitutively activating LH receptor and wild-type LH receptor activated by hCG. Endocrine 25:111-5
Ji, Inhae; Lee, ChangWoo; Jeoung, MyoungKun et al. (2004) Trans-activation of mutant follicle-stimulating hormone receptors selectively generates only one of two hormone signals. Mol Endocrinol 18:968-78
Ko, CheMyong; Grieshaber, Nicole A; Ji, Inhae et al. (2003) Follicle-stimulating hormone suppresses cytosolic 3,5,3'-triiodothyronine-binding protein messenger ribonucleic acid expression in rat granulosa cells. Endocrinology 144:2360-7
Grieshaber, Nicole A; Ko, CheMyong; Grieshaber, Scott S et al. (2003) Follicle-stimulating hormone-responsive cytoskeletal genes in rat granulosa cells: class I beta-tubulin, tropomyosin-4, and kinesin heavy chain. Endocrinology 144:29-39
Sohn, Johann; Youn, HyeSook; Jeoung, MyoungKun et al. (2003) Orientation of follicle-stimulating hormone (FSH) subunits complexed with the FSH receptor. Beta subunit toward the N terminus of exodomain and alpha subunit to exoloop 3. J Biol Chem 278:47868-76
Ji, Inhae; Lee, ChangWoo; Song, YongSang et al. (2002) Cis- and trans-activation of hormone receptors: the LH receptor. Mol Endocrinol 16:1299-308

Showing the most recent 10 out of 58 publications