Abstract

This application seeks to continue dissection of structure-function relationships of the interaction between LH/hCG and the LH/hCG receptor.
Aim 1 concerns analysis of sites on the hormone that contact and activate the receptor, while Aim 2 examines the receptor for sites that contact the hormone.
Aim 3 deals with analysis of receptor domains responsible for delivery of the activation signal to either cAMP or IP signalling pathways. The goal is to define, at the amino acid level, the chemical processes by which hCG binds and activates its receptor, leading to activation of two distinct pathways.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018702-25
Application #
6182177
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Yoshinaga, Koji
Project Start
1992-02-01
Project End
2001-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
25
Fiscal Year
2000
Total Cost
$332,210
Indirect Cost

  Institution

Name
University of Kentucky
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Youn, HyeSook; Ji, Inhae; Ji, Hanlee P et al. (2007) Under-expression of Kalirin-7 Increases iNOS activity in cultured cells and correlates to elevated iNOS activity in Alzheimer's disease hippocampus. J Alzheimers Dis 12:271-81
Jeoung, MyoungKun; Lee, ChangWoo; Ji, Inhae et al. (2007) Trans-activation, cis-activation and signal selection of gonadotropin receptors. Mol Cell Endocrinol 260-262:137-43
Youn, HyeSook; Jeoung, MyoungKun; Koo, YongBum et al. (2007) Kalirin is under-expressed in Alzheimer's disease hippocampus. J Alzheimers Dis 11:385-97
Youn, HyeSook; Koo, YongBum; Ji, Inhae et al. (2005) An upstream initiator-like element suppresses transcription of the rat luteinizing hormone receptor gene. Mol Endocrinol 19:1318-28
Lee, ChangWoo; Ji, Inhae; Ji, Tae H (2004) Distinct mechanisms of cAMP induction by constitutively activating LH receptor and wild-type LH receptor activated by hCG. Endocrine 25:111-5
Ji, Inhae; Lee, ChangWoo; Jeoung, MyoungKun et al. (2004) Trans-activation of mutant follicle-stimulating hormone receptors selectively generates only one of two hormone signals. Mol Endocrinol 18:968-78
Ko, CheMyong; Grieshaber, Nicole A; Ji, Inhae et al. (2003) Follicle-stimulating hormone suppresses cytosolic 3,5,3'-triiodothyronine-binding protein messenger ribonucleic acid expression in rat granulosa cells. Endocrinology 144:2360-7
Grieshaber, Nicole A; Ko, CheMyong; Grieshaber, Scott S et al. (2003) Follicle-stimulating hormone-responsive cytoskeletal genes in rat granulosa cells: class I beta-tubulin, tropomyosin-4, and kinesin heavy chain. Endocrinology 144:29-39
Sohn, Johann; Youn, HyeSook; Jeoung, MyoungKun et al. (2003) Orientation of follicle-stimulating hormone (FSH) subunits complexed with the FSH receptor. Beta subunit toward the N terminus of exodomain and alpha subunit to exoloop 3. J Biol Chem 278:47868-76
Ji, Inhae; Lee, ChangWoo; Song, YongSang et al. (2002) Cis- and trans-activation of hormone receptors: the LH receptor. Mol Endocrinol 16:1299-308

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