This research is built upon three features: (a) immature sperm do not bind to a hyaluronic acid binding surface, and this serves as an initial marker for cells with impaired development; (b) building from the subset of sperm that do bind [apparently normal in that aspect], it is possible to analyze for other potential abnormalities; and (c) taken together, any sperm that is shown to be absent of abnormalities is a """"""""best"""""""" candidate for intracytoplasmic sperm injection (ICSI). The """"""""other potential abnormalities"""""""" include: presence of an abnormal protein termed CP64; shorter tail length; DNA breakdown; and chromosomal aneuploidy. Together these could form an important diagnostic test. From this background, specific aims are developed, namely:
Aim #1 - use of the hyaluronic bead binding to trap sperm for further detailed analysis;
Aim #2 - analysis of tail length as a simple morphologic detection of sperm immaturity;
Aim #3 - analysis of chromosomal abnormalities via comparison of bound vs. non-bound cells;
and Aim #4 - extension of the above to testicular biopsies and analysis of spherical spermatids.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Research Project (R01)
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Reproductive Biology Study Section (REB)
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Rankin, Tracy L
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Yale University
Obstetrics & Gynecology
Schools of Medicine
New Haven
United States
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Sati, Leyla; Bennett, David; Janes, Michael et al. (2015) Next day determination of ejaculatory sperm motility after overnight shipment of semen to remote locations. J Assist Reprod Genet 32:117-25
Ovari, Laszlo; Sati, Leyla; Stronk, Jill et al. (2010) Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies. Fertil Steril 93:2255-61
Prinosilova, Petra; Kruger, Thinus; Sati, Leyla et al. (2009) Selectivity of hyaluronic acid binding for spermatozoa with normal Tygerberg strict morphology. Reprod Biomed Online 18:177-83
Sati, Leyla; Ovari, Laszlo; Bennett, David et al. (2008) Double probing of human spermatozoa for persistent histones, surplus cytoplasm, apoptosis and DNA fragmentation. Reprod Biomed Online 16:570-9
Huszar, Gabor; Jakab, Attila; Sakkas, Denny et al. (2007) Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. Reprod Biomed Online 14:650-63
Zavaczki, Zoltan; Celik-Ozenci, Ciler; Ovari, Laszlo et al. (2006) Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry. Fertil Steril 85:121-7
Huszar, Gabor; Ozkavukcu, Sinan; Jakab, Attila et al. (2006) Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential: selection of sperm for intracytoplasmic sperm injection. Curr Opin Obstet Gynecol 18:260-7
Jakab, Attila; Sakkas, Denny; Delpiano, Elena et al. (2005) Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil Steril 84:1665-73
Huszar, Gabor; Celik-Ozenci, Ciler; Cayli, Sevil et al. (2004) Semen characteristics after overnight shipping: preservation of sperm concentrations, HspA2 ratios, CK activity, cytoplasmic retention, chromatin maturity, DNA integrity, and sperm shape. J Androl 25:593-604
Cayli, Sevil; Sakkas, Denny; Vigue, Lynne et al. (2004) Cellular maturity and apoptosis in human sperm: creatine kinase, caspase-3 and Bcl-XL levels in mature and diminished maturity sperm. Mol Hum Reprod 10:365-72

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