The objective of this application is to understand the molecular mechanisms involved in the hormonal regulation and tissue-specific expression of the GnRHR gene.
The specific aims i nclude 1) determine the physiologic regulation of GnRHR gene transcription and mRNA stability in vivo and in vitro by GnRH, activin, and estrogen; 2) identify and characterize functional cis-DNA elements of the mouse GnRHR gene and identify factors which interact with these elements; 3) identify and characterize cis-DNA elements and factors that mediate GnRH responsiveness; 4) use the 5'-flanking region of the GnRHR gene to direct oncogene expression to the pituitary in an attempt to develop LH- and FSH-producing cell lines; and 5) use GnRHR transgenes to study the regulation by GnRH, activin, and steroid hormones.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Research Project (R01)
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Biochemical Endocrinology Study Section (BCE)
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De Paolo, Louis V
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Brigham and Women's Hospital
United States
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Stamatiades, George A; Kaiser, Ursula B (2018) Gonadotropin regulation by pulsatile GnRH: Signaling and gene expression. Mol Cell Endocrinol 463:131-141
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