The lining epithelium of the mammalian oviduct is a target tissue for the ovarian steroids, and at the time of ovulation consists of two cell types, namely ciliated and secretory cells, which are present in approximately equal proportions. The apical regions of the secretory cells contain secretory granules and the macromolecules present within these granules are released into the oviductal lumen. In the baboon, these macromolecules in reduced form are glycoproteins with a molecular weight of 110-130 kD. These secretions are estradiol-dependent and oviduct-specific. The human oviduct synthesizes and secretes a family of glycoproteins with similar electrophoretic and antigenic properties. The primary objective of this study is to determine if these glycoproteins are essential for, or enhance the early reproductive events which occur within the baboon and human oviduct. The approach taken is to use both molecular and physiological techniques to fulfill this objective. cDNA probes will be obtained from both baboon and human oviductal libraries, sequenced and compared to known sequences to obtain clues to their potential function. The probes will also be used to determine hormonal control and location (cell compartment) of the mRNA. Fluorescent and colloidal-gold techniques will be used to determine if the glycoproteins bind to or interact with oocytes/embryos and/or sperm in vivo. In vitro fertilization and embryo culture techniques will be used to determine if the glycoproteins enhance embryo development and/or the fertilization process. The ultimate goal of these studies is to determine if these oviduct-specific glycoproteins improve embryo viability and their ability to successfully implant within the uterus. The data from the proposed studies should provide basic information on the physiology of the primate oviduct and improve our knowledge relating to fertilization and the oviductal stages of embryo development. These studies may also have important implications for the current procedures used in IVF-ET programs, and could potentially open an area into new immunological contraceptive methods.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD020571-05A1
Application #
3318778
Study Section
Reproductive Biology Study Section (REB)
Project Start
1986-04-01
Project End
1995-11-30
Budget Start
1990-12-27
Budget End
1991-11-30
Support Year
5
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
O'Day-Bowman, M B; Mavrogianis, P A; Minshall, R D et al. (2002) In vivo versus in vitro oviductal glycoprotein (OGP) association with the zona pellucida (ZP) in the hamster and baboon. Mol Reprod Dev 62:248-56
Martus, N S; Verhage, H G; Mavrogianis, P A et al. (1998) Enhancement of bovine oocyte fertilization in vitro with a bovine oviductal specific glycoprotein. J Reprod Fertil 113:323-9
Verhage, H G; Mavrogianis, P A; O'Day-Bowman, M B et al. (1998) Characteristics of an oviductal glycoprotein and its potential role in the fertilization process. Biol Reprod 58:1098-101
Verhage, H G; Fazleabas, A T; Mavrogianis, P A et al. (1997) The baboon oviduct: characteristics of an oestradiol-dependent oviduct-specific glycoprotein. Hum Reprod Update 3:541-52
Verhage, H G; Mavrogianis, P A; Boomsma, R A et al. (1997) Immunologic and molecular characterization of an estrogen-dependent glycoprotein in the rhesus (Macaca mulatta) oviduct. Biol Reprod 57:525-31
Schmidt, A; Mavrogianis, P A; O'Day-Bowman, M B et al. (1997) Species-specific effect of oviductal glycoproteins on hamster sperm binding to hamster oocytes. Mol Reprod Dev 46:201-7
Schmidt, A; Mavrogianis, P A; O'Day-Bowman, M B et al. (1997) Characterization of antibodies generated against a conserved portion of oviductal glycoprotein (OGP) and endogenous hamster OGP and their ability to decrease sperm binding to the zona pellucida in vitro. Am J Reprod Immunol 38:377-83
Verhage, H G; Fazleabas, A T; Mavrogianis, P A et al. (1997) Characteristics of an oviductal glycoprotein and its potential role in fertility control. J Reprod Fertil Suppl 51:217-26
O'Day-Bowman, M B; Mavrogianis, P A; Reuter, L M et al. (1996) Association of oviduct-specific glycoproteins with human and baboon (Papio anubis) ovarian oocytes and enhancement of human sperm binding to human hemizonae following in vitro incubation. Biol Reprod 54:60-9
Jaffe, R C; Arias, E B; O'Day-Bowman, M B et al. (1996) Regional distribution and hormonal control of estrogen-dependent oviduct-specific glycoprotein messenger ribonucleic acid in the baboon (Papio anubis). Biol Reprod 55:421-6

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