Abstract

Dr. Glabe's objective is to elucidate the molecular basis for the species-specific interaction of the sperm and egg surfaces during fertilization in sea urchins.
Aim 1 is to determine the structure/function relationships of binding a sperm adhesive protein.
Aim 2 is to assess the structure/function relationships of an egg surface receptor and how the receptor interacts with bindin.
Aim 3 is to determine the nature of the interaction of bindin with phospholipid bilayers and the role of this interaction in fertilization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD021379-10
Application #
2198251
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1983-01-01
Project End
1998-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
10
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Kamei, N; Swanson, W J; Glabe, C G (2000) A rapidly diverging EGF protein regulates species-specific signal transduction in early sea urchin development. Dev Biol 225:267-76
Mauk, R; Jaworski, D; Kamei, N et al. (1997) Identification of a 97-kDa heat shock protein from S. franciscanus ovaries with 94% amino acid identity to the S. purpuratus egg surface receptor for sperm. Dev Biol 184:31-7
Moy, G W; Mendoza, L M; Schulz, J R et al. (1996) The sea urchin sperm receptor for egg jelly is a modular protein with extensive homology to the human polycystic kidney disease protein, PKD1. J Cell Biol 133:809-17
Hofmann, A; Tai, M; Wong, W et al. (1995) A sparse matrix screen to establish initial conditions for protein renaturation. Anal Biochem 230:8-15
Burdick, D; Soreghan, B; Kwon, M et al. (1992) Assembly and aggregation properties of synthetic Alzheimer's A4/beta amyloid peptide analogs. J Biol Chem 267:546-54
Knauer, M F; Soreghan, B; Burdick, D et al. (1992) Intracellular accumulation and resistance to degradation of the Alzheimer amyloid A4/beta protein. Proc Natl Acad Sci U S A 89:7437-41
DeAngelis, P L; Glabe, C G (1990) Specific recognition of sulfate esters by bindin, a sperm adhesion protein from sea urchins. Biochim Biophys Acta 1037:100-5
DeAngelis, P L; Glabe, C G (1990) Zinc specifically stimulates the selective binding of a peptide analog of bindin to sulfated fucans. Pept Res 3:62-8
Kennedy, L; DeAngelis, P L; Glabe, C G (1989) Analysis of the membrane-interacting domain of the sea urchin sperm adhesive protein bindin. Biochemistry 28:9153-8
DeAngelis, P L; Glabe, C G (1988) Role of basic amino acids in the interaction of bindin with sulfated fucans. Biochemistry 27:8189-94

Showing the most recent 10 out of 11 publications